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BMRB: 16059

2Q2U

Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase (holo form)

Authors

Piserchio, A., Nair, P.A., Shuman, S., Ghose, R.

Assembly

Chv DNA Ligase Monomer

Entity

1. Chv DNA Ligase (polymer, Thiol state: all free), 319 monomers, 36770.60 Da Detail

MGHHHHHHHH HHSSGHIEGR HMAITKPLLA ATLENIEDVQ FPCLATPKID GIRSVKQTQM LSRTFKPIRN SVMNRLLTEL LPEGSDGEIS IEGATFQDTT SAVMTGHKMY NAKFSYYWFD YVTDDPLKKY IDRVEDMKNY ITVHPHILEH AQVKIIPLIP VEINNITELL QYERDVLSKG FEGVMIRKPD GKYKFGRSTL KEGILLKMKQ FKDAEATIIS MTALFKNTNT KTKDNFGYSK RSTHKSGKVE EDVMGSIEVD YDGVVFSIGT GFDADQRRDF WQNKESYIGK MVKFKYFEMG SKDCPRFPVF IGIRHEEDR

2. AMP (non-polymer), 347.221 Da

Total weight

37117.824 Da

Max. entity weight

36770.6 Da

Entity Connection

covalent 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:LYS48:NZ	2:AMP1:P

Source organism

Chlorella virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.1 %, Completeness: 42.3 %, Completeness (bb): 71.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.3 % (1621 of 3829)	26.9 % (537 of 1999)	55.1 % (827 of 1501)	78.1 % (257 of 329)
Backbone	71.3 % (1348 of 1890)	50.3 % (326 of 648)	81.9 % (766 of 935)	83.4 % (256 of 307)
Sidechain	23.1 % (516 of 2236)	15.6 % (211 of 1351)	35.2 % (304 of 863)	4.5 % (1 of 22)
Aromatic	3.6 % (13 of 362)	6.1 % (11 of 181)	1.1 % (2 of 179)	0.0 % (0 of 2)
Methyl	24.8 % (78 of 314)	24.8 % (39 of 157)	24.8 % (39 of 157)

1. Chv DNA Ligase

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Chv DNA Ligase	[U-13C; U-15N; U-2H]	0.3 mM
2	adenosine monophosphate	natural abundance	0.3 mM
3	MES	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	2 mM
6	EDTA	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: 0.44 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: 0.44 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: 0.34 ppm, Outliers: 2 Detail

Release date

2009-01-14

Citation

Sequence-specific 1H N, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase
Piserchio, A., Nair, P.A., Shuman, S., Ghose, R.
Biomol. NMR Assign. (2009), 3, 77-80, PubMed 19636951 , DOI 10.1007/s12104-009-9145-9 , Abstract

Related entities 1. Chv DNA Ligase, : 1 : 1 : 1 : 5 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC