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BMRB: 16079

7TD6

Mouse prion protein fragment mPrP[A113V,A115V,A118V](90-231) at pH 7.0

Authors

Damberger, F.F., Wuthrich, K., Hornemann, S., von Schroetter, C.

Assembly

mPrP90 3AV

Entity

1. mPrP90 3AV (polymer, Thiol state: all disulfide bound), 142 monomers, 16283.02 Da Detail

GQGGGTHNQW NKPSKPKTNL KHVVGVAAVG AVVGGLGGYM LGSAMSRPMI HFGNDWEDRY YRENMYRYPN QVYYRPVDQY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCVTQYQ KESQAYYDGR RS

Formula weight

16283.02 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS90:SG	1:CYS125:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 42.1 %, Completeness (bb): 78.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.1 % (697 of 1654)	19.6 % (169 of 863)	63.4 % (400 of 631)	80.0 % (128 of 160)
Backbone	78.0 % (657 of 842)	48.5 % (142 of 293)	94.2 % (388 of 412)	92.7 % (127 of 137)
Sidechain	16.9 % (159 of 940)	4.7 % (27 of 570)	37.8 % (131 of 347)	4.3 % (1 of 23)
Aromatic	0.0 % (0 of 162)	0.0 % (0 of 81)	0.0 % (0 of 79)	0.0 % (0 of 2)
Methyl	15.5 % (18 of 116)	10.3 % (6 of 58)	20.7 % (12 of 58)

1. mPrP90 3AV

GQGGGTHNQW NKPSKPKTNL KHVVGVAAVG AVVGGLGGYM LGSAMSRPMI HFGNDWEDRY YRENMYRYPN QVYYRPVDQY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCVTQYQ KESQAYYDGR RS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 (±0.2) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	mPrP90_3AV	[U-99% 13C; U-99% 15N]		1.2 (±0.05) mM
2	sodium phosphate buffer	natural abundance		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

Release date

2008-12-21

Citation

Prion protein-detergent micelle interactions studied by NMR in solution
Hornemann, S., von Schroetter, C., Damberger, F.F., Wuthrich, K.
J. Biol. Chem. (2009), 284, 22713-22721, PubMed 19546219 , DOI 10.1074/jbc.M109.000430 , Abstract

Related entities 1. mPrP90 3AV, : 1 : 4 : 108 entities Detail

Interaction partners 1. mPrP90 3AV, : 36 interactors Detail

More details for 36 interactors identified by 12 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC