BMRB: 16184

Mouse Prion Protein (121-231) with Mutations Y225A and Y226A

Authors

Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.

Assembly

Mouse Prion Protein (121-231)

Entity

1. Mouse Prion Protein (121-231) (polymer, Thiol state: all disulfide bound), 114 monomers, 13209.55 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSS

Formula weight

13209.55 Da

Entity Connection

disulfide 1 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 71.1 %, Completeness (bb): 67.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	71.1 % (938 of 1320)	64.0 % (440 of 688)	73.6 % (371 of 504)	99.2 % (127 of 128)
Backbone	67.8 % (460 of 678)	53.4 % (125 of 234)	67.6 % (225 of 333)	99.1 % (110 of 111)
Sidechain	78.0 % (583 of 747)	69.4 % (315 of 454)	90.9 % (251 of 276)	100.0 % (17 of 17)
Aromatic	44.4 % (56 of 126)	28.6 % (18 of 63)	59.7 % (37 of 62)	100.0 % (1 of 1)
Methyl	57.8 % (52 of 90)	15.6 % (7 of 45)	100.0 % (45 of 45)

1. mpp 121-231

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		2 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2KFM, Strand ID: A Detail

---

Release date

2009-05-19

Citation

Prion protein NMR structure from tammar wallaby (Macropus eugenii) shows that the beta2-alpha2 loop is modulated by long-range sequence effects
Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.
J. Mol. Biol. (2009), 389, 833-845, PubMed 19393664 , DOI 10.1016/j.jmb.2009.04.040 , Abstract

Related entities 1. Mouse Prion Protein (121-231), : 1 : 13 : 143 entities Detail

Interaction partners 1. Mouse Prion Protein (121-231), : 36 interactors Detail

Experiments performed 6 experiments Detail