BMRB: 16185

Mouse Prion Protein (121-231) with Mutation V166A

Authors

Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.

Assembly

Mouse Prion Protein (121-231)

Entity

1. Mouse Prion Protein (121-231) (polymer, Thiol state: all disulfide bound), 114 monomers, 13365.69 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPADQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Formula weight

13365.69 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 73.0 %, Completeness (bb): 69.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.0 % (974 of 1334)	66.5 % (463 of 696)	75.1 % (383 of 510)	100.0 % (128 of 128)
Backbone	69.9 % (474 of 678)	56.0 % (131 of 234)	69.7 % (232 of 333)	100.0 % (111 of 111)
Sidechain	79.5 % (605 of 761)	71.9 % (332 of 462)	90.8 % (256 of 282)	100.0 % (17 of 17)
Aromatic	47.2 % (67 of 142)	31.0 % (22 of 71)	62.9 % (44 of 70)	100.0 % (1 of 1)
Methyl	58.3 % (49 of 84)	16.7 % (7 of 42)	100.0 % (42 of 42)

1. mpp 121-231

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPADQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		1.4 mM
2	sodium acetate	[U-100% 2H]		10 mM
3	sodium azide	natural abundance		0.02 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KFO, Strand ID: A Detail

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Release date

2009-05-19

Citation

Prion protein NMR structure from tammar wallaby (Macropus eugenii) shows that the beta2-alpha2 loop is modulated by long-range sequence effects
Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.
J. Mol. Biol. (2009), 389, 833-845, PubMed 19393664 , DOI 10.1016/j.jmb.2009.04.040 , Abstract

Related entities 1. Mouse Prion Protein (121-231), : 1 : 13 : 143 entities Detail

Interaction partners 1. Mouse Prion Protein (121-231), : 36 interactors Detail

Experiments performed 4 experiments Detail