BMRB: 16318

NMR Structure of CBP TAZ2 and Adenoviral E1A Complex

Authors

Ferreon, J.C., Martinez-Yamout, M., Dyson, H., Wright, P.E.

Assembly

CBP TAZ2/Adenoviral E1A Complex

Entity

1. CBP TAZ2 (polymer, Thiol state: free and other bound), 92 monomers, 10505.38 Da Detail

SPQESRRLSI QRCIQSLVHA CQCRNANCSL PSCQKMKRVV QHTKGCKRKT NGGCPVCKQL IALCCYHAKH CQENKCPVPF CLNIKHKLRQ QQ

2. E1A (polymer, Thiol state: not present), 42 monomers, 4463.898 Da Detail

SHMAPEDPNE EAVSQIFPDS VMLAVQEGID LLTFPPAPGS PE

3. ZN (non-polymer), 65.409 × 3 Da

Total weight

15165.504 Da

Max. entity weight

10505.38 Da

Source organism

Homo sapiens , unidentified adenovirus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.7 %, Completeness (bb): 79.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.7 % (1351 of 1558)	95.9 % (794 of 828)	72.5 % (427 of 589)	92.2 % (130 of 141)
Backbone	79.0 % (616 of 780)	95.8 % (250 of 261)	64.0 % (254 of 397)	91.8 % (112 of 122)
Sidechain	93.9 % (852 of 907)	95.2 % (540 of 567)	91.6 % (294 of 321)	94.7 % (18 of 19)
Aromatic	77.4 % (48 of 62)	90.3 % (28 of 31)	64.5 % (20 of 31)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. CBP TAZ2

SPQESRRLSI QRCIQSLVHA CQCRNANCSL PSCQKMKRVV QHTKGCKRKT NGGCPVCKQL IALCCYHAKH CQENKCPVPF CLNIKHKLRQ QQ

2. E1A

SHMAPEDPNE EAVSQIFPDS VMLAVQEGID LLTFPPAPGS PE

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		1.0 ~ 1.5 mM
2	entity_2	[U-100% 15N]		1.0 ~ 1.5 mM
3	D2O	natural abundance		100 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
4	entity_1	[U-100% 13C; U-100% 15N]		1.0 ~ 1.5 mM
5	entity_2	[U-100% 15N]		1.0 ~ 1.5 mM
6	Tris	natural abundance		20 mM
7	NaCl	natural abundance		50 mM
8	D2O	natural abundance		100 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KJE, Strand ID: A, B Detail

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Release date

2009-09-03

Citation

Structural basis for subversion of cellular control mechanisms by the adenoviral E1A oncoprotein
Ferreon, J.C., Martinez-Yamout, M., Dyson, H., Wright, P.E.
Proc. Natl. Acad. Sci. U. S. A. (2009), 106, 13260-13265, PubMed 19651603 , DOI 10.1073/pnas.0906770106 , Abstract

Related entities 1. CBP TAZ2, : 9 : 44 entities Detail

Related entities 2. E1A, : 1 : 2 : 1 entities Detail

Interaction partners 1. CBP TAZ2, : 1 interactors Detail

Interaction partners 2. E1A, : 3 : 1 interactors Detail

Experiments performed 2 experiments Detail