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NMR STRUCTURE OF HUMAN INSULIN MUTANT GLU-B21-D-GLU, HIS-B10 ASP PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES
Authors
Hua, Q.X., Huang, K., Hu, S.Q., Katsoyanni, P., Weiss, M.A.
Assembly
INSULIN A CHAIN, INSULIN B CHAIN
Entity
1. INSULIN A CHAIN (polymer, Thiol state: all disulfide bound), 21 monomers, 2383.696 Da Detail

GIVEQCCTSI CSLYQLENYC N


2. INSULIN B CHAIN (polymer, Thiol state: all disulfide bound), 30 monomers, 3407.868 Da Detail

FVNQHLCGSD LVEALYLVCG XRGFFYTKPT


Total weight
5791.564 Da
Max. entity weight
3407.868 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.1 %, Completeness: 91.8 %, Completeness (bb): 89.9 % Detail

Polymer type: polypeptide(L)

Total1H
All91.8 % (270 of 294)91.8 % (270 of 294)
Backbone89.9 % (89 of 99)89.9 % (89 of 99)
Sidechain92.8 % (181 of 195)92.8 % (181 of 195)
Aromatic87.9 % (29 of 33)87.9 % (29 of 33)
Methyl100.0 % (28 of 28)100.0 % (28 of 28)

1. INSULIN A CHAIN

GIVEQCCTSI CSLYQLENYC N

2. INSULIN B CHAIN

FVNQHLCGSD LVEALYLVCG XRGFFYTKPT

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1INSULIN A CHAINnatural abundance0.5 mM
2INSULIN B CHAINnatural abundance0.5 mM
3H2Onatural abundance90 %
4D2Onatural abundance10 %

Release date
2010-05-18
Citation
An Achilles' heel in an amyloidogenic protein and its repair: insulin fibrillation and therapeutic design
Yang, Y., Petkova, A., Huang, K., Xu, B., Hua, Q., Ye, I., Chu, Y., Hu, S., Phillips, N.B., Whittaker, J., Ismail-Beigi, F., Mackin, R.B., Katsoyannis, P.G., Tycko, R., Weiss, M.A.
J. Biol. Chem. (2010), 285, 10806-10821, PubMed 20106984 , DOI 10.1074/jbc.M109.067850 ,
Related entities 1. INSULIN A CHAIN, : 1 : 110 : 9 : 7 : 74 entities Detail
Related entities 2. INSULIN B CHAIN, : 1 : 1 : 115 : 81 entities Detail
Interaction partners 1. INSULIN A CHAIN, : 1 : 1 interactors Detail
Interaction partners 2. INSULIN B CHAIN, : 6 : 1 interactors Detail
Experiments performed 3 experiments Detail
Keywords FIBRILLATION, HORMONE, HUMAN INSULIN, MUTANT, STABILITY