BMRB: 16392

NMR relaxation data for the beta-lactamase TEM-1

Authors

Gagne, S.M., Savard, P.

Assembly

TEM-1

Entity

1. TEM-1 (polymer, Thiol state: all disulfide bound), 286 monomers, 31442.77 Da Detail

MSIQHFRVAL IPFFAAFCLP VFAHPGTLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

Formula weight

31442.77 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS75:SG	1:CYS121:SG

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Heteronucl. T₁

602 T₁ values in 3 lists
Coherence Sz, Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 303.15 K, pH 6.6 Detail

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Pressure 1 atm, Temperature 303.15 K, pH 6.6

Experiment name 15N-T1

Heteronucl. T₂

602 T₂ values in 3 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 303.15 K, pH 6.6 Detail

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Pressure 1 atm, Temperature 303.15 K, pH 6.6

Experiment name 15N-T2

Heteronucl. NOE

375 NOE values in 2 lists
Value type relative intensities, Field strength (¹H) 500 MHz, 600 MHz, Pressure 1 atm, Temperature 303.15 K, pH 6.6 Detail

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Pressure 1 atm, Temperature 303.15 K, pH 6.6

Experiment name 1H-15N-NOE

Heteronucl. T₁/T₂

602 T₁/T₂ values in 3 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 303.15 K, pH 6.6 Detail

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Pressure 1 atm, Temperature 303.15 K, pH 6.6

Order parameters

182 S² values in 1 lists
Pressure 1 atm, Temperature 303.15 K, pH 6.6 Detail

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Pressure 1 atm, Temperature 303.15 K, pH 6.6

Experiment name 1H-15N-NOE

List #1 order_parameter_list_1

Release date

2009-07-09

Citation

Backbone dynamics of TEM-1 determined by NMR: evidence for a highly ordered protein
Savard, P., Gagne, S.M.
Biochemistry (2006), 45, 11414-11424, PubMed 16981701 , DOI 10.1021/bi060414q , Abstract

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. TEM-1, : 1 : 48 : 109 entities Detail

Interaction partners 1. TEM-1, : 1 interactors Detail

Experiments performed 8 experiments Detail