BMRB: 16394

NMR Evidence for differential phosphorylation-dependent interactions in WT and deltaF508 CFTR

Authors

Kanelis, V., Hudson, R.P., Thibodeau, P.H., Thomas, P.J., Forman-Kay, J.D.

Assembly

deltaF508 CFTR NBD1-RE monomer

Entity

1. deltaF508 CFTR NBD1-RE monomer (polymer, Thiol state: all free), 284 monomers, 31828.74 Da Detail

TTGIIMENVT AFWEEGFGEL LEKVQQSNGD RKHSSDENNV SFSHLCLVGN PVLKNINLNI EKGEMLAITG STGSGKTSLL MLILGELEAS EGIIKHSGRV SFCSQFSWIM PGTIKENIIG VSYDEYRYKS VVKACQLQQD ITKFAEQDNT VLGEGGVTLS GGQRARISLA RAVYKDADLY LLDSPFGYLD VFTEEQVFES CVCKLMANKT RILVTSKMEH LRKADKILIL HQGSSYFYGT FSELQSLRPD FSSKLMGYDT FDQFTEERRS SILTETLRRF SVDD

Formula weight

31828.74 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 43.3 %, Completeness: 14.4 %, Completeness (bb): 29.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	14.4 % (290 of 2017)	9.8 % (168 of 1712)	40.0 % (122 of 305)
Backbone	29.3 % (254 of 867)	22.5 % (132 of 587)	43.6 % (122 of 280)
Sidechain	2.5 % (29 of 1150)	2.6 % (29 of 1125)	0.0 % (0 of 25)
Aromatic	0.0 % (0 of 135)	0.0 % (0 of 133)	0.0 % (0 of 2)
Methyl	4.4 % (7 of 159)	4.4 % (7 of 159)

1. deltaF508 CFTR NBD1-RE

TTGIIMENVT AFWEEGFGEL LEKVQQSNGD RKHSSDENNV SFSHLCLVGN PVLKNINLNI EKGEMLAITG STGSGKTSLL MLILGELEAS EGIIKHSGRV SFCSQFSWIM PGTIKENIIG VSYDEYRYKS VVKACQLQQD ITKFAEQDNT VLGEGGVTLS GGQRARISLA RAVYKDADLY LLDSPFGYLD VFTEEQVFES CVCKLMANKT RILVTSKMEH LRKADKILIL HQGSSYFYGT FSELQSLRPD FSSKLMGYDT FDQFTEERRS SILTETLRRF SVDD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	deltaF508 CFTR NBD1-RE	[U-100% 15N]		175 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	magnesium chloride	natural abundance		5 mM
5	ATP	natural abundance		5 mM
6	DTT	natural abundance		5 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

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Release date

2009-12-13

Citation

NMR evidence for differential phosphorylation-dependent interactions in WT and DeltaF508 CFTR
Kanelis, V., Hudson, R.P., Thibodeau, P.H., Thomas, P.J., Forman-Kay, J.D.
EMBO J. (2010), 29, 263-277, PubMed 19927121 , DOI 10.1038/emboj.2009.329 , Abstract

Related entities 1. deltaF508 CFTR NBD1-RE monomer, : 1 : 159 entities Detail

Interaction partners 1. deltaF508 CFTR NBD1-RE monomer, : 10 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail