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BMRB: 16398

2KND

1H, 13C and 15N Chemical Shift Assignment for the Psb27 Protein of Synechocystis PCC 6803

Authors

Cormann, K., Nowaczyk, M., Ikeuchi, M., Roegner, M., Stoll, R.

Assembly

Psb27

Entity

1. Psb27 (polymer, Thiol state: all free), 110 monomers, 12218.47 Da Detail

CDSGTGLTGN YSQDTLTVIA TLREAIDLPQ DAPNRQEVQD TARGQINDYI SRYRRKGDAG GLKSFTTMQT ALNSLAGYYT SYGARPIPEK LKKRLQLEFT QAERSIERGV

Formula weight

12218.47 Da

Source organism

Synechocystis sp. PCC 6803

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.7 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.7 % (1033 of 1249)	81.5 % (532 of 653)	80.3 % (384 of 478)	99.2 % (117 of 118)
Backbone	96.0 % (626 of 652)	98.7 % (223 of 226)	93.1 % (298 of 320)	99.1 % (105 of 106)
Sidechain	72.3 % (504 of 697)	72.4 % (309 of 427)	70.9 % (183 of 258)	100.0 % (12 of 12)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 34)	0.0 % (0 of 34)
Methyl	84.5 % (98 of 116)	86.2 % (50 of 58)	82.8 % (48 of 58)

1. Psb27

CDSGTGLTGN YSQDTLTVIA TLREAIDLPQ DAPNRQEVQD TARGQINDYI SRYRRKGDAG GLKSFTTMQT ALNSLAGYYT SYGARPIPEK LKKRLQLEFT QAERSIERGV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Psb27	[U-15N]	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium phosphate buffer, pH 6.5	natural abundance	20 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	Psb27	[U-13C; U-15N]	0.9 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %
8	sodium phosphate buffer, pH 6.5	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.51 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.51 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Release date

2009-11-15

Citation

Sequence-specific (1)H, (13)C, and (15)N backbone assignment of Psb27 from Synechocystis PCC 6803
Cormann, K.U., Ikeuchi, M., Rogner, M., Nowaczyk, M.M., Stoll, R.
Biomol. NMR Assign. (2009), 3, 247-249, PubMed 19789994 , DOI 10.1007/s12104-009-9186-0 , Abstract

Related entities 1. Psb27, : 1 : 2 : 21 entities Detail

Interaction partners 1. Psb27, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC