NMR structure of the C-terminal domain of pUL89
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 90.3 % (389 of 431) | 90.3 % (389 of 431) |
Backbone | 97.1 % (133 of 137) | 97.1 % (133 of 137) |
Sidechain | 87.1 % (256 of 294) | 87.1 % (256 of 294) |
Aromatic | 91.9 % (34 of 37) | 91.9 % (34 of 37) |
Methyl | 80.0 % (32 of 40) | 80.0 % (32 of 40) |
1. pUL89-Cter
DQNHIEQPFY LMGRDKALAV EQFISRFNSG YIKASQELVS YTIKLSHDPI EYLLEQIQNL HRVTLAEGSolvent system 50% H2O / 50% acetonitril (v/v), Pressure 1 atm, Temperature 283 K, pH 3.2, Details 15N and 13C labelled amino acids L578, V587, V606, L620, L621 have been incorporated during chemical synthesis
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | pUL89-Cter | natural abundance | 0.8 mM | |
2 | H2O | natural abundance | 50 % | |
3 | acetonitril | natural abundance | 50 % |