BMRB: 16452

NMR structure of the C-terminal domain of pUL89

Authors

Couvreux, A., Hantz, S., Marquant, R., Champier, G., Alain, S., Morellet, N., Bouaziz, S.

Assembly

pUL89

Entity

1. pUL89 (polymer, Thiol state: not present), 68 monomers, 7907.834 Da Detail

DQNHIEQPFY LMGRDKALAV EQFISRFNSG YIKASQELVS YTIKLSHDPI EYLLEQIQNL HRVTLAEG

Formula weight

7907.834 Da

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.3 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.3 % (389 of 431)	90.3 % (389 of 431)
Backbone	97.1 % (133 of 137)	97.1 % (133 of 137)
Sidechain	87.1 % (256 of 294)	87.1 % (256 of 294)
Aromatic	91.9 % (34 of 37)	91.9 % (34 of 37)
Methyl	80.0 % (32 of 40)	80.0 % (32 of 40)

1. pUL89-Cter

DQNHIEQPFY LMGRDKALAV EQFISRFNSG YIKASQELVS YTIKLSHDPI EYLLEQIQNL HRVTLAEG

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Sample

Solvent system 50% H2O / 50% acetonitril (v/v), Pressure 1 atm, Temperature 283 K, pH 3.2, Details 15N and 13C labelled amino acids L578, V587, V606, L620, L621 have been incorporated during chemical synthesis

#	Name	Isotope labeling	Type	Concentration
1	pUL89-Cter	natural abundance		0.8 mM
2	H2O	natural abundance		50 %
3	acetonitril	natural abundance		50 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2KN8, Strand ID: A Detail

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Release date

2012-08-02

Citation

Identification of the interaction domain of the small terminase subunit pUL89 with the large subunit pUL56 of human cytomegalovirus
Thoma, C., Borst, E., Messerle, M., Rieger, M., Hwang, J., Bogner, E.
Biochemistry (2006), 45, 8855-8863, PubMed 16846228 , DOI 10.1021/bi0600796 , Abstract

Related entities 1. pUL89, : 1 : 4 : 15 entities Detail

Experiments performed 6 experiments Detail