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Found 1 Document (0.805 seconds)

BMRB: 16492

2KO8

The Structure of Anti-TRAP

Authors

McElroy, C.A., Gollnick, P., Foster, M.P.

Assembly

Anti-TRAP

Entity

1. Anti-TRAP (polymer, Thiol state: all other bound), 53 monomers, 5677.525 × 3 Da Detail

XVIATDDLEV ACPKCERAGE IEGTPCPACS GKGVILTAQG YTLLDFIQKH LNK

2. ZN (non-polymer, Thiol state: not present), 65.409 × 3 Da

Total weight

17228.8 Da

Max. entity weight

5677.525 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 95.8 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.8 % (548 of 572)	94.6 % (279 of 295)	96.4 % (217 of 225)	100.0 % (52 of 52)
Backbone	98.7 % (302 of 306)	100.0 % (106 of 106)	97.4 % (147 of 151)	100.0 % (49 of 49)
Sidechain	93.6 % (293 of 313)	91.5 % (173 of 189)	96.7 % (117 of 121)	100.0 % (3 of 3)
Aromatic	63.6 % (14 of 22)	63.6 % (7 of 11)	63.6 % (7 of 11)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. Anti-TRAP

XVIATDDLEV ACPKCERAGE IEGTPCPACS GKGVILTAQG YTLLDFIQKH LNK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KO8, Strand ID: A, B, C Detail

Release date

2010-10-17

Citation

Mechanism for pH-dependent gene regulation by amino-terminus-mediated homooligomerization of Bacillus subtilis anti-trp RNA-binding attenuation protein
Sachleben, J.R., McElroy, C.A., Gollnick, P., Foster, M.P.
Proc. Natl. Acad. Sci. U. S. A. (2010), 107, 15385-15390, PubMed 20713740 , DOI 10.1073/pnas.1004981107 , Abstract

Related entities 1. Anti-TRAP, : 1 : 4 : 4 entities Detail

Interaction partners 1. Anti-TRAP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCO

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HNCACB

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D C(CO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D H(CCO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D HCCH-COSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

7 3D 1H-15N TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 2D 1H-13C HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

9 3D HNHA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

10 3D HNHB

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

11 3D HACAHB-COSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

13 3D 1H-15N/13C TIME-SHARED NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

14 2D HCCH-COSY AROMATIC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

15 2D 1H-13C HSQC AROMATIC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
7	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
8	TRIS	[U-2H]	20 mM
9	beta-mercaptoethanol	natural abundance	20 mM
10	ZnCl2	natural abundance	1 mM
11	D2O	natural abundance	100 %

16 2D IPAP

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

17 2D 15N HETERONUCLEAR NOE

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

18 2D 15N HETERONUCLEAR T2

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

19 2D 15N HETERONUCLEAR T1

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 7.5, Details pH 7.5 at RT

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	5.0 ~ 10.0 mM
2	TRIS	[U-2H]	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	ZnCl2	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_16492_2ko8.nef
Input source #2: Coordindates	2ko8.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:1:FME:C	1:2:VAL:N	unknown	unknown	n/a
2:1:FME:C	2:2:VAL:N	unknown	unknown	n/a
3:1:FME:C	3:2:VAL:N	unknown	unknown	n/a
1:12:CYS:SG	4:1:ZN:ZN	unknown	unknown	n/a
1:15:CYS:SG	4:1:ZN:ZN	unknown	unknown	n/a
1:26:CYS:SG	4:1:ZN:ZN	unknown	unknown	n/a
1:29:CYS:SG	4:1:ZN:ZN	unknown	unknown	n/a
2:12:CYS:SG	4:2:ZN:ZN	unknown	unknown	n/a
2:15:CYS:SG	4:2:ZN:ZN	unknown	unknown	n/a
2:26:CYS:SG	4:2:ZN:ZN	unknown	unknown	n/a
2:29:CYS:SG	4:2:ZN:ZN	unknown	unknown	n/a
3:12:CYS:SG	4:3:ZN:ZN	unknown	unknown	n/a
3:15:CYS:SG	4:3:ZN:ZN	unknown	unknown	n/a
3:26:CYS:SG	4:3:ZN:ZN	unknown	unknown	n/a
3:29:CYS:SG	4:3:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
A	1	FME	N-FORMYLMETHIONINE	Assigned chemical shifts, Distance restraints, Torsion angle restraints, Coordinates
B	1	FME	N-FORMYLMETHIONINE	Distance restraints, Torsion angle restraints, Coordinates
C	1	FME	N-FORMYLMETHIONINE	Distance restraints, Torsion angle restraints, Coordinates
D	1	ZN	ZINC ION	None
D	2	ZN	ZINC ION	None
D	3	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
|||||||||||||||||||||||||||||||||||||||||||||||||||||
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
|||||||||||||||||||||||||||||||||||||||||||||||||||||
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
|||||||||||||||||||||||||||||||||||||||||||||||||||||
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Sequence coverage (%)
A	A	53	100.0
B	B	53	100.0
C	C	53	100.0

Content subtype: combined_16492_2ko8.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	15		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	569		100.0 (chain: A, length: 53)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	6040 (1)	noe	100.0 (chain: A, length: 53), 100.0 (chain: B, length: 53), 100.0 (chain: C, length: 53)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	7601 (1)	noe	100.0 (chain: A, length: 53), 100.0 (chain: B, length: 53), 100.0 (chain: C, length: 53)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_5	OK	312 (312)	.	100.0 (chain: A, length: 53), 100.0 (chain: B, length: 53), 100.0 (chain: C, length: 53)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_7	OK	129 (129)	.	81.1 (chain: A, length: 53), 81.1 (chain: B, length: 53), 81.1 (chain: C, length: 53)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 53, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 293
  - ¹³C chemical shifts: 223
  - ¹⁵N chemical shifts: 53
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID
1	FME

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	295	279	94.6
¹³C chemical shifts	225	217	96.4
¹⁵N chemical shifts	53	52	98.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	106	100.0
¹³C chemical shifts	104	100	96.2
¹⁵N chemical shifts	49	49	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	189	173	91.5
¹³C chemical shifts	121	117	96.7
¹⁵N chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	33	100.0
¹³C chemical shifts	33	33	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	7	63.6
¹³C chemical shifts	11	7	63.6

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 53, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 53, Sequence coverage: 100.0%
  - Entity_assembly_ID: C, Chain length: 53, Sequence coverage: 100.0%
- Total number of restraints: 6023
- Weight of restraints: 1.0 (6023)
- Potential type of restraints: square-well-parabolic (6023)
- Range of distance target values: 2.95, 4.05 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
  - Chain_ID: D
    None
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 53, Sequence coverage: 100.0%
    - Entity_assembly_ID: B, Chain length: 53, Sequence coverage: 100.0%
    - Entity_assembly_ID: C, Chain length: 53, Sequence coverage: 100.0%
  - Total number of restraints: 7597
  - Weight of restraints: 1.0 (7597)
  - Potential type of restraints: square-well-parabolic (7597)
  - Range of distance target values: 2.95, 4.05 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
    - Chain_ID: C
    - Chain_ID: D
      None
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 53, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 53, Sequence coverage: 100.0%
  - Entity_assembly_ID: C, Chain length: 53, Sequence coverage: 100.0%
- Total number of restraints: 312
- Total number of restraints per polymer type: 312
- Weight of restraints: 1.0 (312)
- Potential type of restraints: square-well-parabolic (312)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_7

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 53, Sequence coverage: 81.1%

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
  || || |||| ||||||||||| | |||||||||| |||||||||||||  
..IA.DD.EVAC.KCERAGEIEGT.C.ACSGKGVILT.QGYTLLDFIQKHL  
--------10--------20--------30--------40--------50-

Entity_assembly_ID: B, Chain length: 53, Sequence coverage: 81.1%

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
  || || |||| ||||||||||| | |||||||||| |||||||||||||  
..IA.DD.EVAC.KCERAGEIEGT.C.ACSGKGVILT.QGYTLLDFIQKHL  
--------10--------20--------30--------40--------50-

Entity_assembly_ID: C, Chain length: 53, Sequence coverage: 81.1%

--------10--------20--------30--------40--------50---
XVIATDDLEVACPKCERAGEIEGTPCPACSGKGVILTAQGYTLLDFIQKHLNK
  || || |||| ||||||||||| | |||||||||| |||||||||||||  
..IA.DD.EVAC.KCERAGEIEGT.C.ACSGKGVILT.QGYTLLDFIQKHL  
--------10--------20--------30--------40--------50-

Total number of restraints: 129
Potential type of restraints: undefined (129)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None
- Chain_ID: B
  None
- Chain_ID: C
  None

Keywords Protein

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Found 1 Document (0.805 seconds)

BMRB: 16492

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Anti-TRAP, : 1 : 4 : 4 entities Detail

Interaction partners 1. Anti-TRAP, : 1 interactors Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 6 contents Detail