BMRB: 16494

Backbone and side chain 1H chemical shift assignments for acidic fibrobast growth factor

Authors

Pineda-Lucena, A., Jimenez, M., Lozano, R.M., Nieto, J.L., Santoro, J., Rico, M., Gimenez-Gallego, G.

Assembly

aFGF

Entity

1. aFGF (polymer, Thiol state: all free), 132 monomers, 14932.68 Da Detail

KKPKLLYCSN GGHFLRILPD GTVDGTRDRS DQHIQLQLSA ESVGEVYIKS TETGQYLAMD TDGLLYGSQT PNEECLFLER LEENHYNTYI SKKHAEKNWF VGLKKNGSCK RGPRTHYGQK AILFLPLPVS SD

Formula weight

14932.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.6 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.6 % (738 of 815)	90.6 % (738 of 815)
Backbone	96.3 % (260 of 270)	96.3 % (260 of 270)
Sidechain	87.7 % (478 of 545)	87.7 % (478 of 545)
Aromatic	92.2 % (59 of 64)	92.2 % (59 of 64)
Methyl	92.2 % (59 of 64)	92.2 % (59 of 64)

1. aFGF

KKPKLLYCSN GGHFLRILPD GTVDGTRDRS DQHIQLQLSA ESVGEVYIKS TETGQYLAMD TDGLLYGSQT PNEECLFLER LEENHYNTYI SKKHAEKNWF VGLKKNGSCK RGPRTHYGQK AILFLPLPVS SD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	aFGF	natural abundance		1.0 ~ 2.0 mM
2	sodium phosphate	natural abundance		10 mM
3	TSP	natural abundance		0.1 ~ 0.2 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
6	aFGF	natural abundance		1.0 ~ 2.0 mM
7	sodium phosphate	natural abundance		10 mM
8	TSP	natural abundance		0.1 ~ 0.2 mM
9	D2O	natural abundance		100 %

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Release date

2009-09-14

Citation 1

1H-NMR assignment and solution structure of human acidic fibroblast growth factor activated by inositol hexasulfate
Pineda-Lucena, A., Jimenez, M., Nieto, J.L., Santoro, J., Rico, M., Gimenez-Gallego, G.
J. Mol. Biol. (1994), 242, 81-98, PubMed 7521397 , DOI 10.1006/jmbi.1994.1558 , Abstract

Show more 1 citaion

Citation 2

Three-dimensional structure of acidic fibroblast growth factor in solution: effects of binding to a heparin functional analog
Pineda-Lucena, A., Jimenez, M., Lozano, R.M., Nieto, J.L., Santoro, J., Rico, M., Gimenez-Gallego, G.
J. Mol. Biol. (1996), 264, 162-178, PubMed 8950275 , DOI 10.1006/jmbi.1996.0631 , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. aFGF, : 1 : 25 : 7 : 52 : 188 entities Detail

Interaction partners 1. aFGF, : 9 interactors Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail