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(1-77)Apomyoglobin
Authors
Fedyukina, D., Cavagnero, S.
Assembly
(1-77)Apomyoglobin pH 2.40
Entity
1. (1-77)Apomyoglobin pH 2.40 (polymer, Thiol state: not present), 78 monomers, 8819.165 Da Detail

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILK


Formula weight
8819.165 Da
Source organism
Physeter catodon
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 41.8 %, Completeness (bb): 82.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All41.8 % (392 of 937)18.4 % (89 of 483)61.2 % (227 of 371)91.6 % (76 of 83)
Backbone82.0 % (378 of 461)47.7 % (74 of 155)99.6 % (228 of 229)98.7 % (76 of 77)
Sidechain16.0 % (88 of 549) 4.6 % (15 of 328)34.0 % (73 of 215) 0.0 % (0 of 6)
Aromatic 0.0 % (0 of 74) 0.0 % (0 of 37) 0.0 % (0 of 35) 0.0 % (0 of 2)
Methyl 7.5 % (8 of 106) 1.9 % (1 of 53)13.2 % (7 of 53)

1. 1-77 Apomyoglobin

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILK

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 2.40, Details 5 mM CD3COOH in H2O, pH 2.40, 5% D2O


#NameIsotope labelingTypeConcentration
1(1-77)Apomyoglobin[U-99% 13C; U-99% 15N]313 uM
2CD3COOHnatural abundance5 mM
3H2Onatural abundance95 %
4D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.13 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.13 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.15 ppm, Outliers: 1 Detail
Release date
2010-10-31
Citation
Contribution of long-range interactions to the secondary structure of an unfolded globin
Fedyukina, D.V., Rajagopalan, S., Sekhar, A., Fulmer, E.C., Eun, Y., Cavagnero, S.
Biophys. J. (2010), 99, L37-L39, PubMed 20816043 , DOI 10.1016/j.bpj.2010.06.038 ,
Related entities 1. (1-77)Apomyoglobin pH 2.40, : 1 : 40 : 9 : 51 : 99 entities Detail
Interaction partners 1. (1-77)Apomyoglobin pH 2.40, : 2 interactors Detail
Experiments performed 5 experiments Detail