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(1-119)Apomyoglobin
Authors
Fedyukina, D., Cavagnero, S.
Assembly
(1-119)Apomyoglobin pH 2.40
Entity
1. (1-119)Apomyoglobin pH 2.40 (polymer, Thiol state: not present), 120 monomers, 13674.76 Da Detail

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH


Formula weight
13674.76 Da
Source organism
Physeter catodon
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 43.8 %, Completeness (bb): 80.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All43.8 % (639 of 1459)21.8 % (165 of 756)62.5 % (361 of 578)90.4 % (113 of 125)
Backbone80.8 % (572 of 708)48.9 % (116 of 237)97.2 % (344 of 354)95.7 % (112 of 117)
Sidechain20.5 % (177 of 865) 9.4 % (49 of 519)37.6 % (127 of 338)12.5 % (1 of 8)
Aromatic 0.0 % (0 of 120) 0.0 % (0 of 60) 0.0 % (0 of 58) 0.0 % (0 of 2)
Methyl16.7 % (26 of 156)10.3 % (8 of 78)23.1 % (18 of 78)

1. (1-119)Apomyoglobin

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 2.40, Details 5 mM CD3COOH in H2O, pH 2.40, 5% D2O


#NameIsotope labelingTypeConcentration
1(1-119)Apomyoglobin[U-99% 13C; U-99% 15N]280 uM
2CD3COOHnatural abundance5 mM
3H2Onatural abundance95 %
4D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.13 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.13 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.19 ppm, Outliers: 1 Detail
Release date
2010-10-31
Citation
Contribution of long-range interactions to the secondary structure of an unfolded globin
Fedyukina, D.V., Rajagopalan, S., Sekhar, A., Fulmer, E.C., Eun, Y., Cavagnero, S.
Biophys. J. (2010), 99, L37-L39, PubMed 20816043 , DOI 10.1016/j.bpj.2010.06.038 ,
Related entities 1. (1-119)Apomyoglobin pH 2.40, : 1 : 31 : 11 : 58 : 98 entities Detail
Interaction partners 1. (1-119)Apomyoglobin pH 2.40, : 2 interactors Detail
Experiments performed 5 experiments Detail