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(1-153)Apomyoglobin
Authors
Fedyukina, D., Cavagnero, S.
Assembly
(1-153)Apomyoglobin pH 2.40 (ref. DSS)
Entity
1. (1-153)Apomyoglobin pH 2.40 (ref. DSS) (polymer, Thiol state: not present), 154 monomers, 17330.85 Da Detail

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG


Formula weight
17330.85 Da
Source organism
Physeter catodon
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.4 %, Completeness: 40.1 %, Completeness (bb): 76.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.1 % (741 of 1849)19.8 % (190 of 960)56.3 % (409 of 727)87.7 % (142 of 162)
Backbone76.4 % (691 of 905)46.7 % (142 of 304)90.2 % (407 of 451)94.7 % (142 of 150)
Sidechain15.6 % (170 of 1087) 7.3 % (48 of 656)29.1 % (122 of 419) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 156) 0.0 % (0 of 78) 0.0 % (0 of 76) 0.0 % (0 of 2)
Methyl12.0 % (22 of 184) 5.4 % (5 of 92)18.5 % (17 of 92)

1. (1-153)Apomyoglobin

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 2.40, Details 5 mM CD3COOH in H2O, pH 2.40, 5% D2O


#NameIsotope labelingTypeConcentration
1(1-153)Apomyoglobin[U-99% 13C; U-99% 15N]304 uM
2CD3COOHnatural abundance5 mM
3H2Onatural abundance95 %
4D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.23 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.23 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.06 ppm, Outliers: 1 Detail
Release date
2010-10-31
Citation
Contribution of long-range interactions to the secondary structure of an unfolded globin
Fedyukina, D.V., Rajagopalan, S., Sekhar, A., Fulmer, E.C., Eun, Y., Cavagnero, S.
Biophys. J. (2010), 99, L37-L39, PubMed 20816043 , DOI 10.1016/j.bpj.2010.06.038 ,
Related entities 1. (1-153)Apomyoglobin pH 2.40 (ref. DSS), : 1 : 13 : 27 : 60 : 101 entities Detail
Interaction partners 1. (1-153)Apomyoglobin pH 2.40 (ref. DSS), : 2 interactors Detail
Experiments performed 5 experiments Detail