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BMRB: 16537

8XI7

Backbone assignment of the 26 kDa neuron-specific ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)

Authors

Andersson, F.I., Jackson, S.E., Hsu, S.

Assembly

UCH-L1

Entity

1. UCH-L1 (polymer, Thiol state: unknown), 223 monomers, 24824.05 Da Detail

MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA

Formula weight

24824.05 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 48.9 %, Completeness (bb): 85.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.9 % (1270 of 2597)	32.1 % (435 of 1355)	62.5 % (628 of 1004)	87.0 % (207 of 238)
Backbone	85.8 % (1132 of 1320)	70.6 % (319 of 452)	92.8 % (607 of 654)	96.3 % (206 of 214)
Sidechain	21.9 % (325 of 1485)	13.0 % (117 of 903)	37.1 % (207 of 558)	4.2 % (1 of 24)
Aromatic	9.3 % (17 of 182)	18.7 % (17 of 91)	0.0 % (0 of 90)	0.0 % (0 of 1)
Methyl	19.2 % (46 of 240)	14.2 % (17 of 120)	24.2 % (29 of 120)

1. UCH-L1

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
1	UCH-L1	[U-98% 13C; U-98% 15N]	0.2 ~ 0.4 mM
2	TRIS	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	DTT	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Release date

2009-12-17

Citation

Backbone assignments of the 26 kDa neuron-specific ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)
Andersson, F.I., Jackson, S.E., Hsu, S.
Biomol. NMR Assign. (2010), 4, 41-43, PubMed 20012716 , DOI 10.1007/s12104-009-9203-3 , Abstract

Related entities 1. UCH-L1, : 1 : 8 : 3 : 3 : 102 entities Detail

Interaction partners 1. UCH-L1, : 33 interactors Detail

More details for 33 interactors identified by 15 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC