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1H, 15N and 13C assignments of the dimeric C-terminal domain of HIV-1 capsid protein
Authors
Jung, J., Byeon, I.L., Ahn, J., Concel, J., Gronenborn, A.M.
Assembly
CA-CTD dimer
Entity
1. CA-CTD dimer (polymer, Thiol state: all free), 88 monomers, 9827.213 × 2 Da Detail

MYSPTSILDI RQGPKEPFRD YVDRFYKTLR AEQASQEVKN WMTETLLVQN ANPDCKTILK ALGPAATLEE MMTACQGVGG PGHKARVL


Total weight
19654.426 Da
Max. entity weight
9827.213 Da
Source organism
Lentivirus
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 82.9 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All82.9 % (851 of 1027)89.5 % (479 of 535)70.7 % (280 of 396)95.8 % (92 of 96)
Backbone81.8 % (417 of 510)97.6 % (166 of 170)66.3 % (171 of 258)97.6 % (80 of 82)
Sidechain86.0 % (515 of 599)85.8 % (313 of 365)86.4 % (190 of 220)85.7 % (12 of 14)
Aromatic65.0 % (39 of 60)66.7 % (20 of 30)62.1 % (18 of 29)100.0 % (1 of 1)
Methyl94.7 % (89 of 94)95.7 % (45 of 47)93.6 % (44 of 47)

1. HIV-1 Capsid Protein

MYSPTSILDI RQGPKEPFRD YVDRFYKTLR AEQASQEVKN WMTETLLVQN ANPDCKTILK ALGPAATLEE MMTACQGVGG PGHKARVL

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1HIV-1 CA-CTD[U-100% 13C; U-100% 15N]2 mM
2H2Onatural abundance93 %
3D2Onatural abundance7 %
4sodium phosphatenatural abundance25 mM
5sodium azidenatural abundance0.02 %
6DTTnatural abundance2 mM

LACS Plot; CA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.05 ppm, Outliers: 1 Detail
Release date
2009-11-29
Citation
1H, 15N and 13C assignments of the dimeric C-terminal domain of HIV-1 capsid protein
Jung, J., Byeon, I.L., Ahn, J., Concel, J., Gronenborn, A.M.
Biomol. NMR Assign. (2010), 4, 21-23, PubMed 19921549 , DOI 10.1007/s12104-009-9198-9 ,
Related entities 1. CA-CTD dimer, : 1 : 16 : 8 : 98 : 78 entities Detail
Interaction partners 1. CA-CTD dimer, : 1 interactors Detail
Experiments performed 11 experiments Detail
Keywords CA, capsid protein, CTD, C-terminal domain, HIV-1