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Solution structure of SNase140
Authors
Wang, M., Feng, Y., Yao, H., Wang, J.
Assembly
SNase140
Entity
1. SNase140 (polymer, Thiol state: not present), 140 monomers, 15883.27 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW


Formula weight
15883.27 Da
Source organism
Staphylococcus aureus
Exptl. method
SOLUTION NMR
Refine. method
molecular dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.6 %, Completeness: 89.5 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.5 % (1527 of 1707)90.1 % (809 of 898)88.2 % (581 of 659)91.3 % (137 of 150)
Backbone97.6 % (799 of 819)96.7 % (265 of 274)98.3 % (404 of 411)97.0 % (130 of 134)
Sidechain84.1 % (857 of 1019)87.2 % (544 of 624)80.7 % (306 of 379)43.8 % (7 of 16)
Aromatic42.7 % (47 of 110)83.6 % (46 of 55) 0.0 % (0 of 54)100.0 % (1 of 1)
Methyl95.3 % (143 of 150)98.7 % (74 of 75)92.0 % (69 of 75)

1. SNase140

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 K, pH 5.0


#NameIsotope labelingTypeConcentration
1sodium acetate[U-2H]50 mM
2potassium chloridenatural abundance250 mM
3EDTAnatural abundance1 mM
4sodium azidenatural abundance0.02 %
5DSSnatural abundance0.2 mM
6H20natural abundance90 %
7D20natural abundance10 %

LACS Plot; CA
Referencing offset: 0.13 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 0.13 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.2 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2KQ3, Strand ID: A Detail


Release date
2010-05-18
Citation
Importance of the C-terminal loop L137-S141 for the folding and folding stability of staphylococcal nuclease
Wang, M., Feng, Y., Yao, H., Wang, J.
Biochemistry (2010), 49, 4318-4326, PubMed 20415411 , DOI 10.1021/bi100118k ,
Related entities 1. SNase140, : 1 : 8 : 44 : 68 entities Detail
Experiments performed 12 experiments Detail
Keywords Nuclease