BMRB: 16587

Chemical shifts of a native-like folding intermediate of beta2-microglobulin

Authors

Rennella, E., Esposito, G., Corazza, A., Giorgetti, S., Stoppini, M., Bellotti, V.

Assembly

W60G beta2-microglobulin

Entity

1. W60G beta2-microglobulin (polymer, Thiol state: all disulfide bound), 100 monomers, 11733.06 Da Detail

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Formula weight

11733.06 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS26:SG	1:CYS81:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.0 %, Completeness: 56.3 %, Completeness (bb): 73.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	56.3 % (414 of 736)	54.6 % (345 of 632)	66.3 % (69 of 104)
Backbone	73.1 % (215 of 294)	74.4 % (148 of 199)	70.5 % (67 of 95)
Sidechain	45.0 % (199 of 442)	45.5 % (197 of 433)	22.2 % (2 of 9)
Aromatic	60.9 % (39 of 64)	60.3 % (38 of 63)	100.0 % (1 of 1)
Methyl	77.8 % (35 of 45)	77.8 % (35 of 45)

1. W60G beta2-microglobulin

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

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Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 306 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	W60G beta2-microglobulin	[U-100% 15N]		0.6 mM
2	H2O	[U-100% 2H]		0.08 v/v
3	H2O	natural abundance		0.74 v/v
4	TFE	[U-99% 2H]		0.18 v/v
5	sodium chloride	natural abundance		100 mM
6	sodium phosphate	natural abundance		70 mM

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Release date

2010-02-08

Citation 1

Native-unlike long-lived intermediates along the folding pathway of the amyloidogenic protein beta2-microglobulin revealed by real-time two-dimensional NMR
Corazza, A., Rennella, E., Schanda, P., Mimmi, M., Cutuil, T., Raimondi, S., Giorgetti, S., Fogolari, F., Viglino, P., Frydman, L., Gal, M., Bellotti, V., Brutscher, B., Esposito, G.
J. Biol. Chem. (2010), 285, 5827-5835, PubMed 20028983 , DOI 10.1074/jbc.M109.061168 , Abstract

Show more 3 citaions

Citation 2

Folding and fibrillogenesis: clues from beta2-microglobulin in aqueous trifluoroethanol
Rennella, E., Corazza, A., Giorgetti, S., Fogolari, F., Viglino, P., Porcari, R., Stoppini, M., Bellotti, V., Esposito, G.
na

Citation 3

NMR-based characterization of a refolding intermediate of beta2-microglobulin labeled using a wheat germ cell-free system
Kameda, A., Morita, E.H., Sakurai, K., Naiki, H., Goto, Y.
Protein Sci. (2009), 18, 1592-1601, PubMed 19606503 , DOI 10.1002/pro.179 , Abstract

Citation 4

Nuclear magnetic resonance characterization of the refolding intermediate of beta2-microglobulin trapped by non-native prolyl peptide bond
Kameda, A., Hoshino, M., Higurashi, T., Takahashi, S., Naiki, H., Goto, Y.
J. Mol. Biol. (2005), 348, 383-397, PubMed 15811375 , DOI 10.1016/j.jmb.2005.02.050 , Abstract

Hide non-primary 3 citaions

Related entities 1. W60G beta2-microglobulin, : 1 : 1 : 1 : 101 : 185 entities Detail

Interaction partners 1. W60G beta2-microglobulin, : 121 interactors Detail

Experiments performed 4 experiments Detail