BMRB: 16597

Influenza Virus Fusion Peptide in Membranes without Cholesterol at pH 5.0

Authors

Weliky, D.P.

Assembly

Membrane-Associated Influenza Virus Fusion Peptide

Entity

1. Membrane-Associated Influenza Virus Fusion Peptide (polymer, Thiol state: not present), 27 monomers, 2738.125 × 2 Da Detail

GLFGAIAGFI ENGWEGMIDG GGKKKKG

Total weight

5476.25 Da

Max. entity weight

2738.125 Da

Source organism

unidentified influenza virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 55.6 %, Completeness: 49.6 %, Completeness (bb): 51.4 % Detail

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Polymer type: polypeptide(L)

	Total	13C
All	49.6 % (57 of 115)	49.6 % (57 of 115)
Backbone	51.4 % (37 of 72)	51.4 % (37 of 72)
Sidechain	47.5 % (29 of 61)	47.5 % (29 of 61)
Aromatic	66.7 % (10 of 15)	66.7 % (10 of 15)
Methyl	80.0 % (8 of 10)	80.0 % (8 of 10)

1. Influenza Fusion Peptide

GLFGAIAGFI ENGWEGMIDG GGKKKKG

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 233 K, pH 5.0, Details The membrane composition: Di-O-tetradecylphosphatidylcholine:Di-O-tetradecylphosphatidylglycerol (4:1).

#	Name	Isotope labeling	Type	Concentration
1	Influenza Fusion Peptide	[U-99% 13C; U-99% 15N]		20 mM
2	DTPC	natural abundance		400 mM
3	DTPG	natural abundance		100 mM
4	HEPES	natural abundance		10 mM
5	MES	natural abundance		5 mM
6	H2O	natural abundance		27 M

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Release date

2009-11-08

Citation 1

13C-13C correlation spectroscopy of membrane-associated influenza virus fusion peptide strongly supports a helix-turn-helix motif and two turn conformations
Sun, Y., Weliky, D.P.
J. Am. Chem. Soc. (2009), 131, 13228-13229, PubMed 19711890 , DOI 10.1021/ja905198q , Abstract

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Citation 2

13C-13C and (15)N-(13)C correlation spectroscopy of membrane-associated and uniformly labeled human immunodeficiency virus and influenza fusion peptides: amino acid-type assignments and evidence for multiple conformations
Bodner, M.L., Gabrys, C.M., Struppe, J.O., Weliky, D.P.
J. Chem. Phys. (2008), 128, 052319-052319, PubMed 18266436 , DOI 10.1063/1.2829984 , Abstract

Hide non-primary 1 citaion

Related entities 1. Membrane-Associated Influenza Virus Fusion Peptide, : 7 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail