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BMRB: 16599

3KPX

Backbone resonance assignments of clytin

Authors

Feng, Y., Wang, J.

Assembly

clytin monomer

Entity

1. clytin (polymer, Thiol state: all free), 198 monomers, 22425.89 Da Detail

MTDTASKYAV KLKTNFEDPK WVNRHKFMFN FLDINGNGKI TLDEIVSKAS DDICAKLGAT PAQTQRHQEA VEAFFKKIGL DYGKEVEFPA FVNGWKELAK HDLKLWSQNK KSLIRNWGEA VFDIFDKDGS GSISLDEWKT YGGISGICPS DEDAEKTFKH CDLDNSGKLD VDEMTRQHLG FWYTLDPNAD GLYGNFVP

2. CZH (non-polymer), 455.462 Da

Total weight

22881.354 Da

Max. entity weight

22425.89 Da

Source organism

Clytia gregaria

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 61.5 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	61.5 % (1442 of 2343)	52.8 % (643 of 1217)	65.9 % (601 of 912)	92.5 % (198 of 214)
Backbone	94.0 % (1105 of 1176)	92.4 % (375 of 406)	94.5 % (546 of 578)	95.8 % (184 of 192)
Sidechain	37.9 % (511 of 1349)	33.0 % (268 of 811)	44.4 % (229 of 516)	63.6 % (14 of 22)
Aromatic	11.8 % (31 of 262)	13.0 % (17 of 131)	10.4 % (13 of 125)	16.7 % (1 of 6)
Methyl	30.2 % (55 of 182)	30.8 % (28 of 91)	29.7 % (27 of 91)

1. clytin

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	clytin	[U-13C; U-15N]	1.0 mM
2	Tris-HCl	natural abundance	20 mM
3	sodium chloride	natural abundance	10 mM
4	EDTA	natural abundance	2 mM
5	DSS	natural abundance	0.02 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.53 ppm, Outliers: 1 Detail

Release date

2010-09-27

Citation

NMR-derived topology of a GFP-photoprotein energy transfer complex
Titushin, M.S., Feng, Y., Stepanyuk, G.A., Li, Y., Markova, S.V., Golz, S., Wang, B., Lee, J., Wang, J., Vysotski, E.S., Liu, Z.
J. Biol. Chem. (2010), 285, 40891-40900, PubMed 20926380 , DOI 10.1074/jbc.M110.133843 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16600 released on 2010-09-27
Title Backbone resonance assignments of cgGFP

Related entities 1. clytin, : 1 : 131 entities Detail

Interaction partners 1. clytin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC