BMRBj - BMREntryMaster

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BMRB: 16611

2KQS

Phosphorylation of SUMO-interacting motif by CK2 enhances Daxx SUMO binding activity.

Authors

Naik, M.T., Huang, T., Shih, H.

Assembly

SUMO-1 Daxx SIM complex

Entity

1. SUMO1 (polymer, Thiol state: all free), 97 monomers, 11132.40 Da Detail

MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG

2. DAXX20 (polymer, Thiol state: all free), 20 monomers, 2150.317 Da Detail

KTSVATQCDP EEIIVLSDSD

Total weight

13282.717 Da

Max. entity weight

11132.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.9 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.9 % (1296 of 1365)	94.7 % (681 of 719)	94.5 % (494 of 523)	98.4 % (121 of 123)
Backbone	97.8 % (679 of 694)	97.5 % (232 of 238)	97.7 % (335 of 343)	99.1 % (112 of 113)
Sidechain	93.1 % (726 of 780)	93.3 % (449 of 481)	92.7 % (268 of 289)	90.0 % (9 of 10)
Aromatic	57.6 % (38 of 66)	57.6 % (19 of 33)	57.6 % (19 of 33)
Methyl	96.3 % (104 of 108)	96.3 % (52 of 54)	96.3 % (52 of 54)

1. SUMO1

MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG

2. DAXX20

KTSVATQCDP EEIIVLSDSD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 15N]	0.27 ~ 0.5 mM
2	DAXX20	natural abundance	0.0 ~ 1.7 mM
3	potassium phosphate	natural abundance	10 mM
4	potassium chloride	natural abundance	100 mM
5	DTT	natural abundance	2 mM
6	EDTA	natural abundance	0.1 mM
7	sodium azide	natural abundance	0.001 %
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
28	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
29	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
30	potassium phosphate	natural abundance	10 mM
31	potassium chloride	natural abundance	100 mM
32	DTT	natural abundance	2 mM
33	EDTA	natural abundance	0.1 mM
34	sodium azide	natural abundance	0.001 %
35	Pf1 phage	natural abundance	0.0066 w/v
36	H2O	natural abundance	90 %
37	D2O	natural abundance	10 %

Release date

2010-10-13

Citation

NMR chemical shift assignments of a complex between SUMO-1 and SIM peptide derived from the C-terminus of Daxx
Naik, M.T., Chang, C., Naik, N.M., Kung, C., Shih, H., Huang, T.
Biomol. NMR Assign. (2011), 5, 75-77, PubMed 20927612 , DOI 10.1007/s12104-010-9271-4 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9UER7 released on 2002-11-01
Title DAXX_HUMAN Entity Death domain-associated protein 6

Related entities 1. SUMO1, : 3 : 51 entities Detail

Related entities 2. DAXX20, : 1 : 7 : 1 : 1 entities Detail

Interaction partners 2. DAXX20, : 82 interactors Detail

More details for 82 interactors identified by 46 citations

Experiments performed 25 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 15N]	0.27 ~ 0.5 mM
2	DAXX20	natural abundance	0.0 ~ 1.7 mM
3	potassium phosphate	natural abundance	10 mM
4	potassium chloride	natural abundance	100 mM
5	DTT	natural abundance	2 mM
6	EDTA	natural abundance	0.1 mM
7	sodium azide	natural abundance	0.001 %
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

3 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

6 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

8 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

10 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

11 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

12 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

13 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

14 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

15 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

16 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

17 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

18 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

19 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

20 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

21 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
19	SUMO1	natural abundance	2 mM
20	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
21	potassium phosphate	natural abundance	10 mM
22	potassium chloride	natural abundance	100 mM
23	DTT	natural abundance	2 mM
24	EDTA	natural abundance	0.1 mM
25	sodium azide	natural abundance	0.001 %
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

22 3D 1H-15N NOESY - F1 filtered

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

23 3D 1H-13C NOESY - F1 filtered

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

24 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20

#	Name	Isotope labeling	Concentration
10	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
11	DAXX20	natural abundance	2 mM
12	potassium phosphate	natural abundance	10 mM
13	potassium chloride	natural abundance	100 mM
14	DTT	natural abundance	2 mM
15	EDTA	natural abundance	0.1 mM
16	sodium azide	natural abundance	0.001 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

25 2D 1H-15N HSQC - IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20

#	Name	Isotope labeling	Concentration
28	SUMO1	[U-100% 13C; U-100% 15N]	0.5 mM
29	DAXX20	[U-100% 13C; U-100% 15N]	0.5 mM
30	potassium phosphate	natural abundance	10 mM
31	potassium chloride	natural abundance	100 mM
32	DTT	natural abundance	2 mM
33	EDTA	natural abundance	0.1 mM
34	sodium azide	natural abundance	0.001 %
35	Pf1 phage	natural abundance	0.0066 w/v
36	H2O	natural abundance	90 %
37	D2O	natural abundance	10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16611_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16611_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1410		100.0 (chain: 1, length: 97), 100.0 (chain: 2, length: 20)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 100.0%
  - Entity_assembly_ID: 2, Chain length: 20, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 797
  - ¹³C chemical shifts: 493
  - ¹⁵N chemical shifts: 120
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	612	581	94.9
¹³C chemical shifts	440	418	95.0
¹⁵N chemical shifts	106	102	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	199	198	99.5
¹³C chemical shifts	194	193	99.5
¹⁵N chemical shifts	94	94	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	413	383	92.7
¹³C chemical shifts	246	225	91.5
¹⁵N chemical shifts	12	8	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	45	43	95.6
¹³C chemical shifts	45	43	95.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	107	104	97.2
¹³C chemical shifts	83	75	90.4
¹⁵N chemical shifts	20	18	90.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	36	92.3
¹³C chemical shifts	40	32	80.0
¹⁵N chemical shifts	19	17	89.5

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Found 1 Document (2.047 seconds)

BMRB: 16611

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. SUMO1, : 3 : 51 entities Detail

Related entities 2. DAXX20, : 1 : 7 : 1 : 1 entities Detail

Interaction partners 2. DAXX20, : 82 interactors Detail

Experiments performed 25 experiments Detail

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