BMRB: 16663

NMR STRUCTURE OF HUMAN INSULIN MUTANT GLY-B8-D-ALA, HIS-B10-2 ASP PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES

Authors

Hua, Q.X., Wan, Z.L., Zhao, M., Jia, W., Huang, K., Weiss, M.A.

Assembly

HUMAN INSULIN MUTANT

Entity

1. HUMAN INSULIN MUTANT, entity, chain 1 (polymer, Thiol state: all disulfide bound), 21 monomers, 2383.696 Da Detail

GIVEQCCTSI CSLYQLENYC N

2. HUMAN INSULIN MUTANT, entity, chain 2 (polymer, Thiol state: all disulfide bound), 30 monomers, 3421.894 Da Detail

FVNQHLCXSD LVEALYLVCG ERGFFYTKPT

Total weight

5805.59 Da

Max. entity weight

3421.894 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DISTANCE GEOMETRY AND SIMULATED

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 93.6 %, Completeness (bb): 93.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.6 % (278 of 297)	93.6 % (278 of 297)
Backbone	93.1 % (95 of 102)	93.1 % (95 of 102)
Sidechain	93.8 % (183 of 195)	93.8 % (183 of 195)
Aromatic	90.9 % (30 of 33)	90.9 % (30 of 33)
Methyl	96.4 % (27 of 28)	96.4 % (27 of 28)

1. entity, chain 1

GIVEQCCTSI CSLYQLENYC N

2. entity, chain 2

FVNQHLCXSD LVEALYLVCG ERGFFYTKPT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		0.5 ~ 0.8 mM
2	entity_2	natural abundance		0.5 ~ 0.8 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KQQ, Strand ID: A, B Detail

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Release date

2012-08-02

Citation

Insulin: a small protein with a long journey
Hua, Q.
Protein. Cell (2010), 1, 537-551, PubMed 21204007 , DOI 10.1007/s13238-010-0069-z , Abstract

Related entities 1. HUMAN INSULIN MUTANT, entity, chain 1, : 112 : 13 : 7 : 90 entities Detail

Related entities 2. HUMAN INSULIN MUTANT, entity, chain 2, : 1 : 1 : 116 : 97 entities Detail

Interaction partners 1. HUMAN INSULIN MUTANT, entity, chain 1, : 1 : 1 interactors Detail

Interaction partners 2. HUMAN INSULIN MUTANT, entity, chain 2, : 6 : 1 interactors Detail

Experiments performed 3 experiments Detail