BMRB: 16677

Inter-molecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric back-protonation and 2D NOESY

Authors

Nudelman, I., Akabayov, S.R., Schnur, E., Biron, Z., Levy, R., Xu, Y., Yang, D., Anglister, J.

Assembly

interferon-receptor complex

Entity

1. interferon-receptor complex, entity 1 (polymer, Thiol state: all disulfide bound), 165 monomers, 19240.89 Da Detail

CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

2. interferon-receptor complex, entity 2 (polymer, Thiol state: all disulfide bound), 212 monomers, 24299.20 Da Detail

SYDSPDYTDE SCTFKISLRN FRSILSWELK NHSIVPTHYT LLYTIMSKPE DLKVVKNCAN TTRSFCDLTD EWRSTHEAYV TVLEGFSGNT TLFSCSHNFW LAIDMSFEPP EFEIVGFTNH INVMVKFPSI VEEELQFDLS LVIEEQSEGI VKKHKPEIKG NMSGNFTYII DKLIPNTNYC VSVYLEHSDE QAVIKSPLKC TLLPPGQESE FS

Total weight

43540.09 Da

Max. entity weight

24299.2 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS98:SG
2	disulfide	sing	1:CYS29:SG	1:CYS138:SG
3	disulfide	sing	2:CYS12:SG	2:CYS95:SG
4	disulfide	sing	2:CYS58:SG	2:CYS66:SG
5	disulfide	sing	2:CYS180:SG	2:CYS200:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.2 %, Completeness: 63.7 %, Completeness (bb): 72.9 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	15N
All	63.7 % (1753 of 2754)	61.5 % (1449 of 2356)	76.4 % (304 of 398)
Backbone	72.9 % (810 of 1111)	67.6 % (507 of 750)	83.9 % (303 of 361)
Sidechain	57.4 % (943 of 1643)	58.7 % (942 of 1606)	2.7 % (1 of 37)
Aromatic	38.3 % (85 of 222)	39.2 % (85 of 217)	0.0 % (0 of 5)
Methyl	79.9 % (163 of 204)	79.9 % (163 of 204)

1. entity 1

CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

2. entity 2

SYDSPDYTDE SCTFKISLRN FRSILSWELK NHSIVPTHYT LLYTIMSKPE DLKVVKNCAN TTRSFCDLTD EWRSTHEAYV TVLEGFSGNT TLFSCSHNFW LAIDMSFEPP EFEIVGFTNH INVMVKFPSI VEEELQFDLS LVIEEQSEGI VKKHKPEIKG NMSGNFTYII DKLIPNTNYC VSVYLEHSDE QAVIKSPLKC TLLPPGQESE FS

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-2H; NA-H,F,W,Y]		0.25 mM
2	entity_2	[U-2H; NA-V,L,T,M,A,K]		0.25 mM
3	TRIS	[U-2H]		25 mM
4	sodium azide	natural abundance		0.02 %
5	D2O	natural abundance		100 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
6	entity_1	[U-2H; NA-K,R,L,A,M]		0.25 mM
7	entity_2	[U-2H; NA-H,F,W]		0.25 mM
8	TRIS	[U-2H]		25 mM
9	sodium azide	natural abundance		0.02 %
10	D2O	natural abundance		100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
11	entity_1	[U-2H; NA-L]		0.25 mM
12	entity_2	[U-2H; NA-H,W]		0.25 mM
13	TRIS	[U-2H]		25 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	natural abundance		100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
16	entity_1	natural abundance		0.25 mM
17	entity_2	[U-15N]		0.25 mM
18	TRIS	[U-2H]		25 mM
19	sodium azide	natural abundance		0.02 %
20	D2O	natural abundance		100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
21	entity_1	[U-15N]		0.25 mM
22	entity_2	natural abundance		0.25 mM
23	TRIS	[U-2H]		25 mM
24	sodium azide	natural abundance		0.02 %
25	D2O	natural abundance		100 %

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2LAG, Strand ID: A, B Detail

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Release date

2010-05-25

Citation

Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY
Nudelman, I., Akabayov, S.R., Schnur, E., Biron, Z., Levy, R., Xu, Y., Yang, D., Anglister, J.
Biochemistry (2010), 49, 5117-5133, PubMed 20496919 , DOI 10.1021/bi100041f , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. interferon-receptor complex, entity 1, : 1 : 7 : 1 : 138 entities Detail

Related entities 2. interferon-receptor complex, entity 2, : 2 : 9 entities Detail

Interaction partners 1. interferon-receptor complex, entity 1, : 1 interactors Detail

Interaction partners 2. interferon-receptor complex, entity 2, : 12 : 1 interactors Detail

Experiments performed 5 experiments Detail