BMRBj - BMREntryMaster

Found 1 Document (1.463 seconds)

BMRB: 16740

2GC4

Amide assignments for Paracoccus denitrificans amicyanin-ZnII.

Authors

Ubbink, M.

Assembly

Zn-amicyanin

Entity

1. amicyanin (polymer, Thiol state: all other bound), 106 monomers, 11547.09 Da Detail

GDKATIPSES PFAAAEVADG AIVVDIAKMK YETPELHVKV GDTVTWINRE AMPHNVHFVA GVLGEAALKG PMMKKEQAYS LTFTEAGTYD YHCTPHPFMR GKVVVE

2. ZN (non-polymer), 65.409 Da

Total weight

11612.499 Da

Max. entity weight

11547.09 Da

Source organism

Paracoccus denitrificans

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 31.1 %, Completeness (bb): 63.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	31.1 % (222 of 714)	20.6 % (126 of 611)	93.2 % (96 of 103)
Backbone	63.5 % (198 of 312)	48.8 % (104 of 213)	94.9 % (94 of 99)
Sidechain	6.2 % (25 of 402)	5.8 % (23 of 398)	50.0 % (2 of 4)
Aromatic	0.0 % (0 of 53)	0.0 % (0 of 52)	0.0 % (0 of 1)
Methyl	8.2 % (5 of 61)	8.2 % (5 of 61)

1. amicyanin

GDKATIPSES PFAAAEVADG AIVVDIAKMK YETPELHVKV GDTVTWINRE AMPHNVHFVA GVLGEAALKG PMMKKEQAYS LTFTEAGTYD YHCTPHPFMR GKVVVE

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 300 K, pH 7.9

#	Name	Isotope labeling	Type	Concentration
1	amicyanin	[U-15N]		1.2 mM

Release date

2010-10-04

Citation

Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex
Meschi, F., Wiertz, F., Klauss, L., Cavalieri, C., Blok, A., Ludwig, B., Heering, H.A., Merli, A., Rossi, G., Ubbink, M.
J. Am. Chem. Soc. (2010), 132, 14537-14545, PubMed 20873742 , DOI 10.1021/ja105498m , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16741 released on 2010-10-04
Title Backbone assignments for Paracoccus denitrificans amicyanin-CuI.

Related entities 1. amicyanin, : 1 : 31 : 34 entities Detail

Interaction partners 1. amicyanin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16740_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16740_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	194		88.7 (chain: 1, length: 106)
1	Chemical shift references	chemical_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 88.7%
- Total number of assignments
  - ¹H chemical shifts: 98
  - ¹⁵N chemical shifts: 96
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	611	98	16.0
¹⁵N chemical shifts	105	96	91.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	213	94	44.1
¹⁵N chemical shifts	99	94	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	398	4	1.0
¹⁵N chemical shifts	6	2	33.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	66	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords electron transfer, kinetics

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Found 1 Document (1.463 seconds)

BMRB: 16740

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. amicyanin, : 1 : 31 : 34 entities Detail

Interaction partners 1. amicyanin, : 1 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail