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NMR solution structure of a soluble PrgI mutant from Salmonella Typhimurium
Authors
Schmidt, H., Poyraz, O., Seidel, K., Delissen, F., Ader, C., Tenenboim, H., Goosmann, C., Laube, B., Thuenemann, A.F., Zychlinski, A., Baldus, M., Lange, A., Griesinger, C., Kolbe, M.
Assembly
PrgI mutant
Entity
1. PrgI mutant (polymer, Thiol state: not present), 83 monomers, 9081.964 Da Detail

GSHMATPWSG YLDDVSAKFD TGVDNLQTQV TEALDKLAAK PSDPALLAAY QSKLSEYNLY RNAQSNTAKA FKDIDAAIIQ NFR


Formula weight
9081.964 Da
Source organism
Salmonella enterica
Exptl. method
SOLUTION NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.7 % (901 of 941)98.1 % (474 of 483)92.1 % (336 of 365)97.8 % (91 of 93)
Backbone98.4 % (481 of 489)100.0 % (163 of 163)97.6 % (240 of 246)97.5 % (78 of 80)
Sidechain94.0 % (500 of 532)97.2 % (311 of 320)88.4 % (176 of 199)100.0 % (13 of 13)
Aromatic59.0 % (46 of 78)76.9 % (30 of 39)39.5 % (15 of 38)100.0 % (1 of 1)
Methyl100.0 % (92 of 92)100.0 % (46 of 46)100.0 % (46 of 46)

1. PrgI

GSHMATPWSG YLDDVSAKFD TGVDNLQTQV TEALDKLAAK PSDPALLAAY QSKLSEYNLY RNAQSNTAKA FKDIDAAIIQ NFR

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 5.5, Details 0.3 mM PrgI* 20 mM MES 20 mM NaCl pH 5.5


#NameIsotope labelingTypeConcentration
1PrgI*[U-100% 15N]0.3 mM
2MESnatural abundance20 mM
3NaClnatural abundance20 mM
Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 5.5, Details 0.3 mM PrgI* 20 mM MES 20 mM NaCl pH 5.5


#NameIsotope labelingTypeConcentration
4PrgI*[U-100% 13C; U-100% 15N]0.3 mM
5MESnatural abundance20 mM
6NaClnatural abundance20 mM
Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 5.5, Details 0.3 mM PrgI* 20 mM MES 20 mM NaCl pH 5.5 18mg/ml pf1 phages


#NameIsotope labelingTypeConcentration
7PrgI*[U-100% 15N]0.3 mM
8MESnatural abundance20 mM
9NaClnatural abundance20 mM

LACS Plot; CA
Referencing offset: 0.11 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.11 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.14 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.45 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 25 models in PDB: 2KV7, Strand ID: A Detail


Release date
2010-07-26
Citation
Protein refolding is required for assembly of the type three secretion needle
Poyraz, O., Schmidt, H., Seidel, K., Delissen, F., Ader, C., Tenenboim, H., Goosmann, C., Laube, B., Thunemann, A.F., Zychlinsky, A., Baldus, M., Lange, A., Griesinger, C., Kolbe, M.
Nat. Struct. Mol. Biol. (2010), 17, 788-792, PubMed 20543831 , DOI 10.1038/nsmb.1822 ,
Related entities 1. PrgI mutant, : 1 : 1 : 7 : 16 entities Detail
Interaction partners 1. PrgI mutant, : 1 interactors Detail
Experiments performed 14 experiments Detail
Keywords bacterial pathogenesis, needle protomer, NMR solution structure, PrgI, protein transport, type three secretion needle