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BMRB: 16818

2K8A

Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PUBD, Gly76-Pro77 trans isomer)

Authors

Fu, Q., Zhou, C., Gao, H., Lin, D., Hu, H.

Assembly

PFUC trans

Entity

1. PFUC trans (polymer, Thiol state: not present), 80 monomers, 9025.957 Da Detail

ANQQTSGKVL YEGKEFDYVF SIDVNEGGPS YKLPYNTSDD PWLTAYNFLQ KNDLNPMFLD QVAKFIIDNT KGQMLGLGNP

Formula weight

9025.957 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 77.6 %, Completeness (bb): 85.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.6 % (740 of 954)	82.7 % (411 of 497)	65.8 % (242 of 368)	97.8 % (87 of 89)
Backbone	85.3 % (401 of 470)	98.1 % (159 of 162)	72.1 % (168 of 233)	98.7 % (74 of 75)
Sidechain	73.4 % (409 of 557)	75.2 % (252 of 335)	69.7 % (145 of 208)	85.7 % (12 of 14)
Aromatic	16.7 % (17 of 102)	31.4 % (16 of 51)	0.0 % (0 of 50)	100.0 % (1 of 1)
Methyl	83.8 % (62 of 74)	86.5 % (32 of 37)	81.1 % (30 of 37)

1. PFUC trans

ANQQTSGKVL YEGKEFDYVF SIDVNEGGPS YKLPYNTSDD PWLTAYNFLQ KNDLNPMFLD QVAKFIIDNT KGQMLGLGNP

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 20mM phosphate, 50mM NaCl, 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
1	PUBD_trans	[U-100% 13C; U-100% 15N]	1 (±0.02) mM
2	NaCl	natural abundance	50 mM
3	phosphate	natural abundance	20 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	100 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 20mM phosphate, 50mM NaCl, 100% D2O

#	Name	Isotope labeling	Concentration
6	PUBD_trans	[U-100% 13C; U-100% 15N]	1 (±0.02) mM
7	NaCl	natural abundance	50 mM
8	phosphate	natural abundance	20 mM
9	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.03 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.03 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2K8A, Strand ID: A Detail

Release date

2010-05-10

Citation

Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein
Fu, Q., Zhou, C., Gao, H., Jiang, Y., Zhou, Z., Hong, J., Yao, W., Song, A., Lin, D., Hu, H.
J. Biol. Chem. (2009), 284, 19043-19052, PubMed 19423704 , DOI 10.1074/jbc.M109.009126 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 16848 released on 2010-05-09
    Title Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PFUC, Gly76-Pro77 cis isomer).
  Swiss-Prot: Q9Y263 released on 2001-01-11
    Title PLAP_HUMAN Entity Phospholipase A-2-activating protein

Related entities 1. PFUC trans, : 1 : 5 : 4 : 17 entities Detail

Interaction partners 1. PFUC trans, : 19 interactors Detail

More details for 19 interactors identified by 9 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC