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BMRB: 16840

2L9C

1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse

Authors

Phelan, M.M., McLean, L., Simpson, D., Hurst, J.L., Beynon, R.J., Lian, L.

Assembly

darcin

Entity

1. darcin (polymer, Thiol state: all disulfide bound), 176 monomers, 20535.75 Da Detail

MGSSHHHHHH IEGREEASSM ERNFNVEKIN GEWYTIMLAT DKREKIEEHG SMRVFVEYIH VLENSLALKF HIIINEECSE IFLVADKTEK AGEYSVTYDG SNTFTILKTD YDNYIMIHLI NKKDGETFQL MELYGREPDL SSDIKEKFAQ LSEEHGIVRE NIIDLTNANR CLEARE

Formula weight

20535.75 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS78:SG	1:CYS171:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 94.1 %, Completeness (bb): 94.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.1 % (1960 of 2083)	93.6 % (1013 of 1082)	94.3 % (766 of 812)	95.8 % (181 of 189)
Backbone	94.9 % (1000 of 1054)	94.4 % (340 of 360)	95.0 % (493 of 519)	95.4 % (167 of 175)
Sidechain	93.5 % (1118 of 1196)	93.2 % (673 of 722)	93.7 % (431 of 460)	100.0 % (14 of 14)
Aromatic	80.2 % (146 of 182)	80.2 % (73 of 91)	80.0 % (72 of 90)	100.0 % (1 of 1)
Methyl	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (91 of 91)

1. darcin

MGSSHHHHHH IEGREEASSM ERNFNVEKIN GEWYTIMLAT DKREKIEEHG SMRVFVEYIH VLENSLALKF HIIINEECSE IFLVADKTEK AGEYSVTYDG SNTFTILKTD YDNYIMIHLI NKKDGETFQL MELYGREPDL SSDIKEKFAQ LSEEHGIVRE NIIDLTNANR CLEARE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	darcin	[U-98% 13C; U-98% 15N]	1 mM
2	potassium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.2 %
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	darcin	[U-98% 13C; U-98% 15N]	1 mM
7	potassium phosphate	natural abundance	25 mM
8	sodium azide	natural abundance	0.2 %
9	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.7358 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.7358 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.7358 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.7358 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L9C, Strand ID: A Detail

Release date

2010-08-18

Citation

1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein
Phelan, M.M., McLean, L., Simpson, D.M., Hurst, J.L., Beynon, R.J., Lian, L.
Biomol. NMR Assign. (2010), 4, 239-241, PubMed 20703836 , DOI 10.1007/s12104-010-9253-6 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17447 released on 2012-08-29
Title 1H, 15N and 13C resonance assignment of a common Major Urinary Protein of the mouse