BMRBj - BMREntryMaster

Found 1 Document (0.821 seconds)

BMRB: 16848

2K89

Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PFUC, Gly76-Pro77 cis isomer).

Authors

Fu, Q.

Assembly

PFUC

Entity

1. PFUC (polymer, Thiol state: not present), 80 monomers, 9025.957 Da Detail

ANQQTSGKVL YEGKEFDYVF SIDVNEGGPS YKLPYNTSDD PWLTAYNFLQ KNDLNPMFLD QVAKFIIDNT KGQMLGLGNP

Formula weight

9025.957 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 74.5 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.5 % (711 of 954)	79.9 % (397 of 497)	61.4 % (226 of 368)	98.9 % (88 of 89)
Backbone	82.1 % (386 of 470)	96.9 % (157 of 162)	66.1 % (154 of 233)	100.0 % (75 of 75)
Sidechain	70.7 % (394 of 557)	71.6 % (240 of 335)	67.8 % (141 of 208)	92.9 % (13 of 14)
Aromatic	15.7 % (16 of 102)	29.4 % (15 of 51)	0.0 % (0 of 50)	100.0 % (1 of 1)
Methyl	83.8 % (62 of 74)	86.5 % (32 of 37)	81.1 % (30 of 37)

1. PFUC-cis

ANQQTSGKVL YEGKEFDYVF SIDVNEGGPS YKLPYNTSDD PWLTAYNFLQ KNDLNPMFLD QVAKFIIDNT KGQMLGLGNP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PFUC	natural abundance	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Release date

2010-05-09

Citation

Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein
Fu, Q., Zhou, C., Gao, H., Jiang, Y., Zhou, Z., Hong, J., Yao, W., Song, A., Lin, D., Hu, H.
J. Biol. Chem. (2009), 284, 19043-19052, PubMed 19423704 , DOI 10.1074/jbc.M109.009126 , Abstract

Related entities 1. PFUC, : 1 : 5 : 4 : 17 entities Detail

Interaction partners 1. PFUC, : 19 interactors Detail

More details for 19 interactors identified by 9 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16848_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16848_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	771		98.8 (chain: 1, length: 80)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 80, Sequence coverage: 98.8%
- Total number of assignments
  - ¹H chemical shifts: 462
  - ¹³C chemical shifts: 221
  - ¹⁵N chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	497	398	80.1
¹³C chemical shifts	368	221	60.1
¹⁵N chemical shifts	89	88	98.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	162	158	97.5
¹³C chemical shifts	160	79	49.4
¹⁵N chemical shifts	75	75	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	335	240	71.6
¹³C chemical shifts	208	142	68.3
¹⁵N chemical shifts	14	13	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	32	82.1
¹³C chemical shifts	39	30	76.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	15	29.4
¹³C chemical shifts	50	0	0.0
¹⁵N chemical shifts	1	1	100.0

Keywords PFU, PFUC, PLAA, Ubiquitin

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Found 1 Document (0.821 seconds)

BMRB: 16848

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PFUC, : 1 : 5 : 4 : 17 entities Detail

Interaction partners 1. PFUC, : 19 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail