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NMR Structure of AIRE PHD Finger
Authors
Chakravarty, S., Zeng, L., ZHOU, M.
Assembly
Reecognition of H3 by the PHD Finger
Entity
1. AIRE PHD finger 1 (polymer, Thiol state: free and other bound), 56 monomers, 6013.776 Da Detail

GSKNEDECAV CRDGGELICC DGCPRAFHLA CLSPPLREIP SGTWRCSSCL QATVQE


2. Histone H3 1-20Cys (polymer, Thiol state: all free), 21 monomers, 2286.658 Da Detail

ARTKQTARKS TGGKAPRKQL C


3. ZN (non-polymer, Thiol state: not present), 65.409 × 2 Da
Total weight
8431.252 Da
Max. entity weight
6013.776 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 70.1 %, Completeness: 57.4 %, Completeness (bb): 56.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All57.4 % (484 of 843)62.1 % (274 of 441)51.4 % (163 of 317)55.3 % (47 of 85)
Backbone56.2 % (250 of 445)67.8 % (101 of 149)46.0 % (103 of 224)63.9 % (46 of 72)
Sidechain60.7 % (284 of 468)59.2 % (173 of 292)67.5 % (110 of 163) 7.7 % (1 of 13)
Aromatic84.6 % (22 of 26)84.6 % (11 of 13)83.3 % (10 of 12)100.0 % (1 of 1)
Methyl75.0 % (48 of 64)75.0 % (24 of 32)75.0 % (24 of 32)

1. AIRE PHD finger 1

GSKNEDECAV CRDGGELICC DGCPRAFHLA CLSPPLREIP SGTWRCSSCL QATVQE

2. Histone H3 1-20Cys

ARTKQTARKS TGGKAPRKQL C

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 10mM phosphate buffer, pH 7.0; 250mM NaCl,


#NameIsotope labelingTypeConcentration
1AIRE PHD finger 1[U-100% 15N]0.5 mM
2phosphate buffernatural abundance10 mM
3NaClnatural abundance250 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.49 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.49 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2KFT, Strand ID: A, B Detail


Release date
2010-07-26
Citation
Structure and site-specific recognition of histone H3 by the PHD finger of human autoimmune regulator
Chakravarty, S., Zeng, L., Zhou, M.
Structure (2009), 17, 670-679, PubMed 19446523 , DOI 10.1016/j.str.2009.02.017 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: O43918 released on 1998-07-15
    Title AIRE_HUMAN Entity Autoimmune regulator
Related entities 1. AIRE PHD finger 1, : 1 : 3 : 159 entities Detail
Related entities 2. Histone H3 1-20Cys, : 1 : 159 : 13 entities Detail
Interaction partners 1. AIRE PHD finger 1, : 13 : 1 interactors Detail
Interaction partners 2. Histone H3 1-20Cys, : 2 interactors Detail
Experiments performed 7 experiments Detail
Keywords AIRE, APECED, Histone Code, PHD Finger, Transcription