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Solution Structure of the Ubiquitin-Binding Motif of Human Polymerase Iota
Authors
Bomar, M.G., D'Souza, S., Bienko, M., Dikic, I., Walker, G.
Assembly
Ubiquitin-Binding Motif
Entity
1. Ubiquitin-Binding Motif (polymer, Thiol state: all free), 108 monomers, 12329.40 Da Detail

MQYKLILNGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTEGSDE KITFPSDIDP QVFYELPEAV QKELLAEWKR TGSDFHIGHK LEHHHHHH


Formula weight
12329.4 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.4 %, Completeness: 88.5 %, Completeness (bb): 93.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All88.5 % (1113 of 1257)89.5 % (578 of 646)86.9 % (431 of 496)90.4 % (104 of 115)
Backbone93.4 % (593 of 635)93.0 % (198 of 213)93.4 % (296 of 317)94.3 % (99 of 105)
Sidechain84.6 % (612 of 723)87.8 % (380 of 433)81.1 % (227 of 280)50.0 % (5 of 10)
Aromatic62.3 % (86 of 138)76.8 % (53 of 69)46.3 % (31 of 67)100.0 % (2 of 2)
Methyl99.1 % (107 of 108)100.0 % (54 of 54)98.1 % (53 of 54)

1. Ubiquitin-Binding Motif

MQYKLILNGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTEGSDE KITFPSDIDP QVFYELPEAV QKELLAEWKR TGSDFHIGHK LEHHHHHH

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1UBM2[U-100% 13C; U-100% 15N]1.0 ~ 4.0 mM
2D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: -0.02 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.02 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.03 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 25 models in PDB: 2KHU, Strand ID: A Detail


Release date
2010-06-14
Citation
Unconventional ubiquitin recognition by the ubiquitin-binding motif within the Y family DNA polymerases iota and Rev1
Bomar, M.G., Bienko, S., Dikic, M., Walker, I., Zhou, G.C.
Mol. Cell (2010), 37, 408-417, PubMed 20159559 , DOI 10.1016/j.molcel.2009.12.038 ,
Related entities 1. Ubiquitin-Binding Motif, : 1 : 1 : 7 entities Detail
Experiments performed 8 experiments Detail
Keywords TLS, UBM, polymerase iota, translesion synthesis, ubiquitin-binding domain