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BMRB: 16895

2KX0

The solution structure of UBB+1, frameshift mutant of ubiquitin B

Authors

Lee, W., Ko, S.

Assembly

UBB+1 (ubiquitin B mutant)

Entity

1. UBB+1 (ubiquitin B mutant) (polymer, Thiol state: not present), 103 monomers, 11521.82 Da Detail

GSLVPRGSMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSEYNIQ KESTLHLVLR LRGYADLRED PDRQDHHPGS GAQ

Formula weight

11521.82 Da

Source organism

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 86.9 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (1042 of 1199)	87.0 % (550 of 632)	86.1 % (396 of 460)	89.7 % (96 of 107)
Backbone	95.5 % (579 of 606)	95.7 % (200 of 209)	94.7 % (284 of 300)	97.9 % (95 of 97)
Sidechain	80.6 % (554 of 687)	82.7 % (350 of 423)	79.9 % (203 of 254)	10.0 % (1 of 10)
Aromatic	25.0 % (12 of 48)	50.0 % (12 of 24)	0.0 % (0 of 24)
Methyl	97.4 % (111 of 114)	98.2 % (56 of 57)	96.5 % (55 of 57)

1. UBB+1

GSLVPRGSMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSEYNIQ KESTLHLVLR LRGYADLRED PDRQDHHPGS GAQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UBB+1	[U-99% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		50 mM
5	NaCl	natural abundance		100 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
10	UBB+1	[U-99% 13C; U-99% 15N]		1 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	NaPO4	natural abundance		50 mM
14	NaCl	natural abundance		100 mM

LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KX0, Strand ID: A Detail

Release date

2011-05-01

Related entities 1. UBB+1 (ubiquitin B mutant), : 10 entities Detail

Experiments performed 8 experiments Detail

1 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

2 3D HNCO

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

3 3D HNCA

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

4 3D HNCACB

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

5 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	UBB+1	[U-100% 13C]		1 mM
7	D2O	natural abundance		100 %
8	NaPO4	natural abundance		50 mM
9	NaCl	natural abundance		100 mM

6 3D 1H-15N NOESY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UBB+1	[U-99% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		50 mM
5	NaCl	natural abundance		100 mM

7 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
10	UBB+1	[U-99% 13C; U-99% 15N]		1 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	NaPO4	natural abundance		50 mM
14	NaCl	natural abundance		100 mM

8 3D 1H-13C NOESY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
10	UBB+1	[U-99% 13C; U-99% 15N]		1 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	NaPO4	natural abundance		50 mM
14	NaCl	natural abundance		100 mM

nullKeywords alzheimer disease, E2-25K, frame shift mutant, protein, ubiquitin