BMRB: 16898

Backbone 1H, 13C and 15N Chemical Shift Assignments for the alpha chain of human haemoglobin bound to alpha-haemoglobin stabilizing protein (AHSP)

Authors

Dickson, C.F., Gell, D.A.

Assembly

alpha-haemoglobin:AHSP protein complex

Entity

1. HBA1 (polymer, Thiol state: all free), 141 monomers, 15126.15 Da Detail

VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R

2. AHSP (polymer, Thiol state: not present), 102 monomers, 11840.26 Da Detail

MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS

Total weight

26966.41 Da

Max. entity weight

15126.15 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 49.0 %, Completeness: 14.8 %, Completeness (bb): 26.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	14.8 % (419 of 2826)	11.3 % (164 of 1456)	12.6 % (141 of 1120)	45.6 % (114 of 250)
Backbone	26.4 % (378 of 1430)	26.4 % (127 of 481)	19.0 % (137 of 720)	49.8 % (114 of 229)
Sidechain	3.2 % (52 of 1630)	3.8 % (37 of 975)	2.4 % (15 of 634)	0.0 % (0 of 21)
Aromatic	2.0 % (5 of 248)	2.4 % (3 of 124)	1.6 % (2 of 122)	0.0 % (0 of 2)
Methyl	3.7 % (11 of 294)	3.4 % (5 of 147)	4.1 % (6 of 147)

1. HBA1

VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R

2. AHSP

MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS

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Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HBA1	[U-98% 13C; U-98% 15N]		0.6 ~ 1.0 mM
2	AHSP	natural abundance		0.6 ~ 1.0 mM
3	D2O	natural abundance		7 %
4	DSS	natural abundance		40 uM
5	H2O	natural abundance		93 %
6	sodium phosphate	natural abundance		20 mM

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Release date

2010-05-26

Citation

α-Hemoglobin-stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-α-hemoglobin and weakens the iron-oxygen bond
Dickson, C.F., Rich, A.M., Collins, W.M.H., Lowry, D.A.T., Mollan, J.A., Khandros, T.L., Olson, E., Weiss, J.S., Mackay, M.J., Lay, J.P., Gell, P.A.
J. Biol. Chem. (2013), 288, 19986-20001, PubMed 23696640 , DOI 10.1074/jbc.M112.437509 , Abstract

Related entities 1. HBA1, : 2 : 3 : 56 entities Detail

Related entities 2. AHSP, : 1 : 2 : 7 : 7 entities Detail

Interaction partners 2. AHSP, : 6 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail