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BMRB: 16900

2LY4

MDM4 binds ligands via an induced fit mechanism in which disordered regions become structured

Authors

Sanchez, M.C., Renshaw, J.G., Davies, G.O., Barlow, P.N., Vogtherr, M.

Assembly

MDM4-p53 complex

Entity

1. MDM4 N-terminal domain (polymer, Thiol state: all free), 102 monomers, 11432.17 Da Detail

GSHMDSASRI SPGQINQVRP KLPLLKILHA AGAQGEMFTV KEVMHYLGQY IMVKQLYDQQ EQHMVYCGGD LLGELLGRQS FSVKDPSPLY DMLRKNLVTL AT

2. p53 peptide (polymer, Thiol state: not present), 11 monomers, 1348.541 Da Detail

ETFSDLWKLL P

Total weight

12780.711 Da

Max. entity weight

11432.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.6 %, Completeness: 36.9 %, Completeness (bb): 71.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.9 % (498 of 1350)	16.3 % (115 of 705)	54.9 % (289 of 526)	79.0 % (94 of 119)
Backbone	71.0 % (473 of 666)	42.8 % (98 of 229)	85.5 % (282 of 330)	86.9 % (93 of 107)
Sidechain	13.8 % (109 of 788)	3.4 % (16 of 476)	30.7 % (92 of 300)	8.3 % (1 of 12)
Aromatic	0.0 % (0 of 98)	0.0 % (0 of 49)	0.0 % (0 of 48)	0.0 % (0 of 1)
Methyl	6.9 % (9 of 130)	3.1 % (2 of 65)	10.8 % (7 of 65)

1. MDM4 N-terminal domain

GSHMDSASRI SPGQINQVRP KLPLLKILHA AGAQGEMFTV KEVMHYLGQY IMVKQLYDQQ EQHMVYCGGD LLGELLGRQS FSVKDPSPLY DMLRKNLVTL AT

2. p53 peptide

ETFSDLWKLL P

Show sample condition

Sample

Solvent system 99% H2O / 1% DMSO, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	MDM4 N-terminal domain	[U-100% 13C; U-100% 15N]	0.1 mM
2	p53 peptide	natural abundance	0.2 (±0.1) mM
3	potassium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.1 %
5	BisTris	natural abundance	50 mM
6	TCEP	natural abundance	2 mM
7	EDTA	natural abundance	0.1 mM
8	H20	natural abundance	99 %
9	DMSO	natural abundance	1 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 4 Detail

Release date

2010-06-15

Citation

MDM4 binds ligands via a mechanism in which disordered regions become structured
Sanchez, M.C., Renshaw, J.G., Davies, G., Barlow, P.N., Vogtherr, M.
FEBS Lett. (2010), 584, 3035-3041, PubMed 20515689 , DOI 10.1016/j.febslet.2010.05.058 , Abstract

Related entities 1. MDM4 N-terminal domain, : 1 : 5 entities Detail

Related entities 2. p53 peptide, : 1 : 20 : 3 entities Detail

Interaction partners 2. p53 peptide, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC