BMRB: 1704

Two-Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from Hydrogen-1, Carbon-13, and Nitrogen-15 Spin System Assignments of the Aromatic Amino Acids in the Nuclease H124L-Thymidine 3',5'-Bisphosp

Authors

Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.

Assembly

micrococcal nuclease

Entity

1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Formula weight

16214.55 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 14.7 %, Completeness: 14.1 %, Completeness (bb): 8.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	14.1 % (243 of 1729)	14.9 % (136 of 912)	15.1 % (101 of 669)	4.1 % (6 of 148)
Backbone	8.6 % (73 of 846)	9.0 % (26 of 289)	9.8 % (41 of 420)	4.4 % (6 of 137)
Sidechain	18.4 % (187 of 1017)	17.7 % (110 of 623)	20.1 % (77 of 383)	0.0 % (0 of 11)
Aromatic	96.4 % (106 of 110)	98.2 % (54 of 55)	96.3 % (52 of 54)	0.0 % (0 of 1)
Methyl	4.0 % (6 of 150)	4.0 % (3 of 75)	4.0 % (3 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Show sample condition

Sample

Temperature 318 K, pH 5.5

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Release date

1995-07-30

Citation

Two-dimensional NMR studies of staphylococcal nuclease: evidence for conformational heterogeneity from hydrogen-1, carbon-13, and nitrogen-15 spin system assignments of the aromatic amino acids in the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Biochemistry (1990), 29, 4242-4253, PubMed 2361141 , DOI: , Abstract

Related entities 1. micrococcal nuclease, : 1 : 8 : 43 : 87 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail