BMRBj - BMREntryMaster

Found 1 Document (1.503 seconds)

BMRB: 17065

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Binary Tandem Ubiquitin Binding Domains of Signal Transducing Adapter Molecule 1

Authors

Ahn, H., Lim, J., Hong, Y., Lee, B.

Assembly

STAM1 VHS-UIM

Entity

1. STAM1 VHS-UIM (polymer, Thiol state: all free), 191 monomers, 21007.85 Da Detail

MPLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA EQAKASPALV AKDPGTVANK KEEEDLAKAI ELSLKEQRQQ S

Formula weight

21007.85 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 45.7 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.7 % (1004 of 2196)	23.8 % (273 of 1148)	65.4 % (553 of 845)	87.7 % (178 of 203)
Backbone	80.2 % (905 of 1128)	52.6 % (201 of 382)	93.3 % (526 of 564)	97.8 % (178 of 182)
Sidechain	21.7 % (271 of 1250)	9.4 % (72 of 766)	43.0 % (199 of 463)	0.0 % (0 of 21)
Aromatic	0.0 % (0 of 100)	0.0 % (0 of 50)	0.0 % (0 of 48)	0.0 % (0 of 2)
Methyl	21.0 % (44 of 210)	14.3 % (15 of 105)	27.6 % (29 of 105)

1. Tandem ubiquitin binding domains of STAM1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Tandem ubiquitin binding domains of STAM1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium phosphate	natural abundance	50 mM
5	NaCl	natural abundance	.1 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Release date

2010-10-13

Citation

Backbone 1H, 13C, and 15N assignments for the tandem ubiquitin binding domains of signal transducing adapter molecule 1
Lim, J., Hong, Y., Lee, B., Ahn, H.
Biomol. NMR Assign. (2011), 5, 51-54, PubMed 20927613 , DOI 10.1007/s12104-010-9265-2 , Abstract

Related entities 1. STAM1 VHS-UIM, : 2 : 54 entities Detail

Interaction partners 1. STAM1 VHS-UIM, : 17 interactors Detail

More details for 17 interactors identified by 10 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17065_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17065_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	877		99.0 (chain: 1, length: 191)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 191, Sequence coverage: 99.0%
- Total number of assignments
  - ¹³C chemical shifts: 523
  - ¹H chemical shifts: 177
  - ¹⁵N chemical shifts: 177
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	111	19	17.1
¹H chemical shifts	111	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	845	523	61.9
¹H chemical shifts	1148	177	15.4
¹⁵N chemical shifts	209	177	84.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	382	352	92.1
¹H chemical shifts	382	177	46.3
¹⁵N chemical shifts	182	177	97.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	463	171	36.9
¹H chemical shifts	766	0	0.0
¹⁵N chemical shifts	27	0	0.0

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Found 1 Document (1.503 seconds)

BMRB: 17065

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. STAM1 VHS-UIM, : 2 : 54 entities Detail

Interaction partners 1. STAM1 VHS-UIM, : 17 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail