BMRB: 17081

Mouse Prion Protein (121-231) with mutation Y169G

Authors

Christen, B., Damberger, F.F., Perez, D.R., Hornemann, S., Wuthrich, K.

Assembly

Prion with Y169G mutation

Entity

1. Prion with Y169G mutation (polymer, Thiol state: all disulfide bound), 114 monomers, 13287.62 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ GSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Formula weight

13287.62 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.6 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.6 % (1283 of 1328)	98.0 % (679 of 693)	94.1 % (477 of 507)	99.2 % (127 of 128)
Backbone	98.4 % (667 of 678)	97.0 % (228 of 235)	99.1 % (329 of 332)	99.1 % (110 of 111)
Sidechain	95.4 % (719 of 754)	98.5 % (451 of 458)	90.0 % (251 of 279)	100.0 % (17 of 17)
Aromatic	79.1 % (106 of 134)	98.5 % (66 of 67)	59.1 % (39 of 66)	100.0 % (1 of 1)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. Prion with Y169G mutation

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ GSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	prion	[U-99% 13C; U-99% 15N]		1.9 mM
2	sodium acetate	[U-2H]		10 mM
3	sodium azide	natural abundance		0.02 %
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.34 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.48 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1D, Strand ID: A Detail

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Release date

2012-03-08

Citation

Cellular prion protein conformation and function
Damberger, F.F., Christen, B., Perez, D.R., Hornemann, S., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 17308-17313, PubMed 21987789 , DOI 10.1073/pnas.1106325108 , Abstract

Related entities 1. Prion with Y169G mutation, : 1 : 13 : 142 entities Detail

Interaction partners 1. Prion with Y169G mutation, : 36 interactors Detail

Experiments performed 2 experiments Detail