BMRB: 17165

Prion-like conversion during amyloid formation at atomic resolution

Authors

Eichner, T., Kalverda, A.P., Thompson, G.S., Radford, S.E., Homans, S.W.

Assembly

WT

Entity

1. WT (polymer, Thiol state: all free), 100 monomers, 11862.21 Da Detail

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Formula weight

11862.21 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

NONE

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.6 %, Completeness (bb): 97.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.6 % (1180 of 1222)	97.2 % (621 of 639)	96.2 % (460 of 478)	94.3 % (99 of 105)
Backbone	97.3 % (574 of 590)	98.5 % (195 of 198)	97.0 % (288 of 297)	95.8 % (91 of 95)
Sidechain	96.4 % (703 of 729)	96.6 % (426 of 441)	96.8 % (269 of 278)	80.0 % (8 of 10)
Aromatic	89.9 % (124 of 138)	92.8 % (64 of 69)	88.1 % (59 of 67)	50.0 % (1 of 2)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. WT

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 (±0.100) atm, Temperature 298.000 (±1.000) K, pH 7.500 (±0.100), Details backbone assignment, 15N_noesy

#	Name	Isotope labeling	Type	Concentration
1	WT	[U-100% 13C; U-100% 15N]		0.5 (±5.0E-5) mM
2	sodium phosphate	natural abundance		25.0 (±0.0025) mM
3	sodium acide	natural abundance		1.0 (±1.0E-4) mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1.000 (±0.100) atm, Temperature 298.000 (±1.000) K, pH 7.500 (±0.100), Details side chain assignment, 13C_noesy

#	Name	Isotope labeling	Type	Concentration
6	WT	[U-100% 13C; U-100% 15N]		0.3 (±3.0E-5) mM
7	sodium phosphate	natural abundance		25.0 (±0.0025) mM
8	sodium acide	natural abundance		1.0 (±1.0E-4) mM
9	D2O	natural abundance		100 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; CO

Referencing offset: 0.96 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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Protein Blocks Logo

Calculated from 30 models in PDB: 2XKS, Strand ID: A Detail

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Release date

2011-02-03

Citation 1

Conformational conversion during amyloid formation at atomic resolution
Eichner, T., Kalverda, A.P., Thompson, G.S., Homans, S.W., Radford, S.E.
Mol. Cell (2011), 41, 161-172, PubMed 21255727 , DOI 10.1016/j.molcel.2010.11.028 , Abstract

Show more 1 citaion

Citation 2

A generic mechanism of beta2-microglobulin amyloid assembly at neutral pH involving a specific proline switch
Eichner, T., Radford, S.E.
J. Mol. Biol. (2009), 386, 1312-1326, PubMed 19452600 , Abstract

Hide non-primary 1 citaion

Related entities 1. WT, : 100 : 4 : 184 entities Detail

Interaction partners 1. WT, : 121 interactors Detail

Experiments performed 19 experiments Detail