BMRB: 17174

MOUSE PRION PROTEIN FRAGMENT 121-231 AT 37 C

Authors

Christen, B., Damberger, F.F., Perez, D.R., Hornemann, S., Wuthrich, K.

Assembly

Mouse prion protein

Entity

1. Mouse prion protein (polymer, Thiol state: all disulfide bound), 114 monomers, 13393.75 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Formula weight

13393.75 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.8 %, Completeness (bb): 84.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (1201 of 1338)	98.9 % (690 of 698)	74.8 % (383 of 512)	100.0 % (128 of 128)
Backbone	84.5 % (573 of 678)	97.9 % (229 of 234)	70.0 % (233 of 333)	100.0 % (111 of 111)
Sidechain	95.8 % (733 of 765)	99.4 % (461 of 464)	89.8 % (255 of 284)	100.0 % (17 of 17)
Aromatic	78.9 % (112 of 142)	98.6 % (70 of 71)	58.6 % (41 of 70)	100.0 % (1 of 1)
Methyl	95.3 % (82 of 86)	95.3 % (41 of 43)	95.3 % (41 of 43)

1. Mouse prion

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.2 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	Mouse_prion	[U-99% 13C; U-99% 15N]		1.6 mM
2	sodium acetate	[U-2H]		10 mM
3	sodium azide	natural abundance		0.02 %
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L39, Strand ID: A Detail

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Release date

2012-03-08

Citation

Cellular prion protein conformation and function
Damberger, F.F., Christen, B., Perez, D.R., Hornemann, S., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 17308-17313, PubMed 21987789 , DOI 10.1073/pnas.1106325108 , Abstract

Related entities 1. Mouse prion protein, : 1 : 1 : 8 : 12 : 135 entities Detail

Interaction partners 1. Mouse prion protein, : 36 interactors Detail

Experiments performed 3 experiments Detail