BMRB: 17201

Backbone assignment of the little finger domain of Y-family DNA polymerase Dpo4

Authors

Ma, D., Fowler, J.

Assembly

little finger domain of Dpo4

Entity

1. little finger domain of Dpo4 (polymer, Thiol state: all free), 131 monomers, 15612.76 Da Detail

MDEYNEPIRT RVRKSIGRIV TMKRNSRNLE EIKPYLFRAI EESYYKLDKR IPKAIHVVAV TEDLDIVSRG RTFPHGISKE TAYSESVKLL QKILEEDERK IRRIGVRFSK FIEAIGLDKF FDTLEHHHHH H

Formula weight

15612.76 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 29.1 %, Completeness (bb): 57.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	29.1 % (472 of 1622)	16.6 % (142 of 855)	33.5 % (213 of 636)	89.3 % (117 of 131)
Backbone	57.3 % (446 of 778)	47.1 % (124 of 263)	52.8 % (205 of 388)	92.1 % (117 of 127)
Sidechain	11.0 % (107 of 970)	3.0 % (18 of 592)	23.8 % (89 of 374)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 132)	0.0 % (0 of 66)	0.0 % (0 of 66)
Methyl	3.4 % (5 of 146)	0.0 % (0 of 73)	6.8 % (5 of 73)

1. Dpo4

MDEYNEPIRT RVRKSIGRIV TMKRNSRNLE EIKPYLFRAI EESYYKLDKR IPKAIHVVAV TEDLDIVSRG RTFPHGISKE TAYSESVKLL QKILEEDERK IRRIGVRFSK FIEAIGLDKF FDTLEHHHHH H

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Dpo4	[U-100% 13C; U-100% 15N]		0.4 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %
4	NaCl	natural abundance		80.0 ~ 200.0 mM
5	phosphate buffer	natural abundance		20 mM

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LACS Plot; CA

Referencing offset: 0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.51 ppm	internal	indirect	0.2514495
1H	water	protons	4.51 ppm	internal	direct	1.0
15N	water	protons	4.51 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.51 ppm	internal	indirect	0.2514495
1H	water	protons	4.51 ppm	internal	direct	1.0
15N	water	protons	4.51 ppm	internal	indirect	0.1013291

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Release date

2011-08-02

Citation

Backbone assignment of the little finger domain of a Y-family DNA polymerase
Ma, D., Fowler, J.D., Suo, Z.
Biomol. NMR Assign. (2011), 5, 195-198, PubMed 21337030 , DOI 10.1007/s12104-011-9298-1 , Abstract

Related entities 1. little finger domain of Dpo4, : 1 : 95 : 16 entities Detail

Interaction partners 1. little finger domain of Dpo4, : 1 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail