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C-terminal zinc knuckle of the HIVNCp7
Authors
Quintal, S.M.O., Viegas, A.J.M., Cabrita, E.J., Farrell, N.P., Erhardt, S.
Assembly
ZNF
Entity
1. C-terminal zinc knuckle of the HIVNCp7 (polymer, Thiol state: all other bound), 19 monomers, 2224.563 Da Detail

KGCWKCGKEG HQMKDCTER


2. ZN (non-polymer), 65.409 × 4 Da
Total weight
2486.199 Da
Max. entity weight
2224.563 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS3:SG2:ZN1:ZN
2nasing1:CYS6:SG2:ZN1:ZN
3nasing1:CYS16:SG2:ZN1:ZN
4nasing1:HIS11:NE22:ZN1:ZN

Source organism
Human immunodeficiency virus
Exptl. method
SOLUTION NMR
Refine. method
DGSA-distance geometry simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.7 %, Completeness: 90.9 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

Total1H
All90.9 % (110 of 121)90.9 % (110 of 121)
Backbone94.7 % (36 of 38)94.7 % (36 of 38)
Sidechain89.2 % (74 of 83)89.2 % (74 of 83)
Aromatic100.0 % (8 of 8)100.0 % (8 of 8)
Methyl100.0 % (1 of 1)100.0 % (1 of 1)

1. C-terminal zinc knuckle of the HIVNCp7

KGCWKCGKEG HQMKDCTER

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7


#NameIsotope labelingTypeConcentration
1C-terminal zinc knuckle of the HIVNCp7natural abundance1 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2L44, Strand ID: A Detail


Release date
2012-03-08
Citation
Platinated DNA affects zinc finger conformation. Interaction of a platinated single-stranded oligonucleotide and the C-terminal zinc finger of nucleocapsid protein HIVNCp7
Quintal, S., Viegas, A., Erhardt, S., Cabrita, E.J., Farrell, N.P.
Biochemistry (2012), 51, 1752-1761, PubMed 22303928 , DOI 10.1021/bi201834g ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 17229 released on 2012-03-08
    Title C-terminal zinc knuckle of the HIVNCp7 with DNA
Related entities 1. C-terminal zinc knuckle of the HIVNCp7, : 1 : 47 : 31 : 5 : 46 entities Detail
Interaction partners 1. C-terminal zinc knuckle of the HIVNCp7, : 1 interactors Detail
Experiments performed 4 experiments Detail
Keywords HIV, NCP7, NMR, Platinum, Zinc finger, Zinc