BMRB: 17228

C-terminal zinc knuckle of the HIVNCp7

Authors

Quintal, S.M.O., Viegas, A.J.M., Cabrita, E.J., Farrell, N.P., Erhardt, S.

Assembly

ZNF

Entity

1. C-terminal zinc knuckle of the HIVNCp7 (polymer, Thiol state: all other bound), 19 monomers, 2224.563 Da Detail

KGCWKCGKEG HQMKDCTER

2. ZN (non-polymer), 65.409 × 4 Da

Total weight

2486.199 Da

Max. entity weight

2224.563 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS3:SG	2:ZN1:ZN
2	na	sing	1:CYS6:SG	2:ZN1:ZN
3	na	sing	1:CYS16:SG	2:ZN1:ZN
4	na	sing	1:HIS11:NE2	2:ZN1:ZN

Source organism

Human immunodeficiency virus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 90.9 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.9 % (110 of 121)	90.9 % (110 of 121)
Backbone	95.1 % (39 of 41)	95.1 % (39 of 41)
Sidechain	88.7 % (71 of 80)	88.7 % (71 of 80)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. C-terminal zinc knuckle of the HIVNCp7

KGCWKCGKEG HQMKDCTER

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	C-terminal zinc knuckle of the HIVNCp7	natural abundance		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Calculated from 20 models in PDB: 2L44, Strand ID: A Detail

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Release date

2012-03-08

Citation

Platinated DNA affects zinc finger conformation. Interaction of a platinated single-stranded oligonucleotide and the C-terminal zinc finger of nucleocapsid protein HIVNCp7
Quintal, S., Viegas, A., Erhardt, S., Cabrita, E.J., Farrell, N.P.
Biochemistry (2012), 51, 1752-1761, PubMed 22303928 , DOI 10.1021/bi201834g , Abstract

Related entities 1. C-terminal zinc knuckle of the HIVNCp7, : 1 : 47 : 31 : 5 : 110 entities Detail

Interaction partners 1. C-terminal zinc knuckle of the HIVNCp7, : 1 interactors Detail

Experiments performed 4 experiments Detail