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C-terminal zinc knuckle of the HIVNCp7 with DNA
Authors
Quintal, S.MO., Viegas, A.JM., Cabrita, E.J., Farrell, N.P., Erhardt, S.
Assembly
ZNF DNA
Entity
1. C-terminal zinc knuckle of the HIVNCp7 (polymer, Thiol state: all other bound), 19 monomers, 2224.563 Da Detail

KGCWKCGKEG HQMKDCTER


2. DNA (polymer, Thiol state: not present), 6 monomers, 1832.166 Da Detail

TACGCC


3. ZN (non-polymer, Thiol state: not present), 65.409 × 4 Da
Total weight
4318.365 Da
Max. entity weight
2224.563 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS3:SG3:ZN1:ZN
2nasing1:CYS6:SG3:ZN1:ZN
3nasing1:CYS16:SG3:ZN1:ZN
4nasing1:HIS11:NE23:ZN1:ZN

Source organism
Human immunodeficiency virus
Exptl. method
SOLUTION NMR
Refine. method
DGSA-distance geometry simulated annealing
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 79.1 %, Completeness (bb): 76.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

Total1H
All79.1 % (144 of 182)79.1 % (144 of 182)
Backbone76.2 % (61 of 80)76.2 % (61 of 80)
Sidechain81.4 % (83 of 102)81.4 % (83 of 102)
Aromatic85.0 % (17 of 20)85.0 % (17 of 20)
Methyl100.0 % (2 of 2)100.0 % (2 of 2)

1. C-terminal zinc knuckle of the HIVNCp7

KGCWKCGKEG HQMKDCTER

2. DNA

TACGCC

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7


#NameIsotope labelingTypeConcentration
1C-terminal zinc knuckle of the HIVNCp7natural abundance1 mM
2DNAnatural abundance1 mM
3H2Onatural abundance90 %
4D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 19 models in PDB: 2L45, Strand ID: A Detail


Release date
2012-03-08
Citation
Platinated DNA affects zinc finger conformation. Interaction of a platinated single-stranded oligonucleotide and the C-terminal zinc finger of nucleocapsid protein HIVNCp7
Quintal, S., Viegas, A., Erhardt, S., Cabrita, E.J., Farrell, N.P.
Biochemistry (2012), 51, 1752-1761, PubMed 22303928 , DOI 10.1021/bi201834g ,
Related entities 1. C-terminal zinc knuckle of the HIVNCp7, : 1 : 47 : 31 : 5 : 46 entities Detail
Experiments performed 4 experiments Detail
Keywords HIV, NCP7, NMR, Platinum, Zinc finger, Zinc