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Solution structures of human PIWI-like 1 PAZ domain
Authors
Zeng, L., Zhang, Q., Yan, K., Zhou, M.
Assembly
Human PIWI-like 1 PAZ domain
Entity
1. Human PIWI-like 1 PAZ domain (polymer, Thiol state: not available), 134 monomers, 15651.57 Da Detail

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND


Formula weight
15651.57 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
DGSA-distance geometry simulated annealing, torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 82.4 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All82.4 % (1350 of 1638)87.2 % (747 of 857)73.6 % (465 of 632)92.6 % (138 of 149)
Backbone82.8 % (648 of 783)98.9 % (258 of 261)67.1 % (265 of 395)98.4 % (125 of 127)
Sidechain84.2 % (827 of 982)82.0 % (489 of 596)89.3 % (325 of 364)59.1 % (13 of 22)
Aromatic59.3 % (83 of 140)60.0 % (42 of 70)58.0 % (40 of 69)100.0 % (1 of 1)
Methyl100.0 % (132 of 132)100.0 % (66 of 66)100.0 % (66 of 66)

1. Human PIWI-like 1 PAZ domain

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1sodium phosphatenatural abundance100 mM
2sodium chloridenatural abundance150 mM
3DTT[U-100% 2H]5 mM
4D2Onatural abundance100 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
5sodium phosphatenatural abundance100 mM
6sodium chloridenatural abundance150 mM
7DTT[U-100% 2H]5 mM
8D2Onatural abundance10 %
9H2Onatural abundance90 %

LACS Plot; CA
Referencing offset: -0.02 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.02 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.04 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2L5C, Strand ID: A Detail


Release date
2011-04-07
Citation
Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain
Zeng, L., Zhang, Q., Yan, K., Zhou, M.
Proteins (2011), 79, 2004-2009, PubMed 21465557 , DOI 10.1002/prot.23003 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 17273 released on 2011-04-07
    Title Solution Structures of human PIWI-like 1 PAZ domain with ssRNA (5'-pUGACA)
Related entities 1. Human PIWI-like 1 PAZ domain, : 1 : 2 : 3 : 20 entities Detail
Interaction partners 1. Human PIWI-like 1 PAZ domain, : 12 interactors Detail
Experiments performed 5 experiments Detail
Keywords PAZ, PIWI, piRNA, ssRNA