BMRB: 17273

Solution Structures of human PIWI-like 1 PAZ domain with ssRNA (5'-pUGACA)

Authors

Zeng, L., Zhang, Q., Yan, K., Zhou, M.

Assembly

human PIWI-like 1 PAZ domain with ssRNA (5'-pUGACA)

Entity

1. PAZ domain (polymer, Thiol state: not available), 134 monomers, 15651.57 Da Detail

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND

2. RNA (polymer, Thiol state: not present), 5 monomers, 1420.885 Da Detail

UGAXA

Total weight

17072.455 Da

Max. entity weight

15651.57 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 81.5 %, Completeness (bb): 80.6 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C	15N
All	81.5 % (1380 of 1693)	85.1 % (757 of 890)	73.7 % (486 of 659)	95.1 % (137 of 144)
Backbone	80.6 % (672 of 834)	90.8 % (265 of 292)	68.0 % (282 of 415)	98.4 % (125 of 127)
Sidechain	84.5 % (833 of 986)	82.3 % (492 of 598)	88.7 % (329 of 371)	70.6 % (12 of 17)
Aromatic	57.6 % (91 of 158)	58.2 % (46 of 79)	57.9 % (44 of 76)	33.3 % (1 of 3)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. PAZ domain

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND

2. RNA

UGAXA

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		100 mM
2	sodium chloride	natural abundance		150 mM
3	DTT	[U-100% 2H]		5 mM
4	D2O	natural abundance		100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	sodium phosphate	natural abundance		100 mM
6	sodium chloride	natural abundance		150 mM
7	DTT	[U-100% 2H]		5 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L5D, Strand ID: A Detail

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Release date

2011-04-07

Citation

Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain
Zeng, L., Zhang, Q., Yan, K., Zhou, M.
Proteins (2011), 79, 2004-2009, PubMed 21465557 , DOI 10.1002/prot.23003 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. PAZ domain, : 1 : 3 : 3 : 44 entities Detail

Experiments performed 5 experiments Detail