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BMRB: 17352

2L7E

The structure of a domain from yeast

Authors

Zhang, W., Zhang, J., Tu, X.

Assembly

Taf14 YEATS domain

Entity

1. Taf14 YEATS domain (polymer, Thiol state: not present), 131 monomers, 15359.33 Da Detail

MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI PLNLEHHHHH H

Formula weight

15359.33 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 66.3 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.3 % (1073 of 1618)	66.5 % (561 of 844)	62.9 % (404 of 642)	81.8 % (108 of 132)
Backbone	84.6 % (645 of 762)	86.3 % (220 of 255)	81.7 % (317 of 388)	90.8 % (108 of 119)
Sidechain	54.9 % (539 of 982)	57.9 % (341 of 589)	52.1 % (198 of 380)	0.0 % (0 of 13)
Aromatic	1.2 % (2 of 164)	1.2 % (1 of 82)	1.3 % (1 of 80)	0.0 % (0 of 2)
Methyl	67.1 % (106 of 158)	81.0 % (64 of 79)	53.2 % (42 of 79)

1. a domain

MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI PLNLEHHHHH H

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
6	a_domain	natural abundance		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		100 mM
9	D2O	natural abundance		100 %

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.99 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L7E, Strand ID: A Detail

Release date

2011-03-29

Citation

Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae
Zhang, W., Zhang, J., Zhang, X., Xu, C., Tu, X.
Biochem. J. (2011), 436, 83-90, PubMed 21355849 , DOI 10.1042/BJ20110004 , Abstract

Related entities 1. Taf14 YEATS domain, : 1 : 11 : 6 entities Detail

Interaction partners 1. Taf14 YEATS domain, : 92 interactors Detail

More details for 92 interactors identified by 20 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 3D C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 3D HNCO

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

8 3D HN(CO)CA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	a_domain	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

9 3D HCCH-TOCSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
6	a_domain	natural abundance		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		100 mM
9	D2O	natural abundance		100 %

10 3D HCCH-COSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
6	a_domain	natural abundance		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		100 mM
9	D2O	natural abundance		100 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_17352_2l7e.nef
Input source #2: Coordindates	2l7e.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERK

-------110-------120-------130-
IPHDLNFLQESYEVEHVIQIPLNLEHHHHHH
|||||||||||||||||||||||||||||||
IPHDLNFLQESYEVEHVIQIPLNLEHHHHHH

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	131	0	0	100.0

Content subtype: combined_17352_2l7e.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1058		90.8 (chain: A, length: 131)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1680 (1)	noe	92.4 (chain: A, length: 131)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	92 (1)	hbond	38.2 (chain: A, length: 131)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	119 (119)	.	69.5 (chain: A, length: 131)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 90.8%
- Total number of assignments
  - ¹³C chemical shifts: 392
  - ¹H chemical shifts: 559
  - ¹⁵N chemical shifts: 107
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 92.4%
- Total number of restraints: 1680
- Weight of restraints: 1.0 (1680)
- Potential type of restraints: square-well-parabolic (1680)
- Range of distance target values: 2.4, 4.4 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 38.2%
  - Total number of restraints: 92
  - Weight of restraints: 1.0 (92)
  - Potential type of restraints: square-well-parabolic (92)
  - Range of distance target values: 2.5, 2.5 (Å)
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 69.5%
- Total number of restraints: 119
- Total number of restraints per polymer type: 119
- Weight of restraints: 1.0 (119)
- Potential type of restraints: square-well-parabolic (119)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords cell growth