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BMRB: 17369

Fyn SH2 bound form

Authors

Huculeci, R.I., Buts, L., Lenaerts, T., van Nuland, N.A.J.

Assembly

MS2

Entity

1. FynSH2 correct (polymer, Thiol state: all free), 112 monomers, 13230.85 Da Detail

NHKVHHHHHH MEWYFGKLGR KDAERQLLSF GNPRGTFLIR ESETTKGAYS LSIRDWDDMK GDHVKHYKIR KLDNGGYYIT TRAQFETLQQ LVQHYSERAA GLCCRLVVPC HK

2. peptide (polymer, Thiol state: not present), 13 monomers, 1497.560 Da Detail

XEPQXEEIPI YLX

Total weight

14728.41 Da

Max. entity weight

13230.85 Da

Source organism

Homo sapiens , Cricetinae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.6 %, Completeness: 82.5 %, Completeness (bb): 86.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.5 % (1222 of 1482)	81.9 % (636 of 777)	82.3 % (474 of 576)	86.8 % (112 of 129)
Backbone	86.0 % (623 of 724)	86.7 % (216 of 249)	85.4 % (305 of 357)	86.4 % (102 of 118)
Sidechain	79.9 % (696 of 871)	79.5 % (420 of 528)	80.1 % (266 of 332)	90.9 % (10 of 11)
Aromatic	71.3 % (117 of 164)	73.2 % (60 of 82)	68.8 % (55 of 80)	100.0 % (2 of 2)
Methyl	89.1 % (98 of 110)	89.1 % (49 of 55)	89.1 % (49 of 55)

1. FynSH2 correct

NHKVHHHHHH MEWYFGKLGR KDAERQLLSF GNPRGTFLIR ESETTKGAYS LSIRDWDDMK GDHVKHYKIR KLDNGGYYIT TRAQFETLQQ LVQHYSERAA GLCCRLVVPC HK

2. peptide

XEPQXEEIPI YLX

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	FynSH2_correct	[U-98% 13C; U-98% 15N]	0.8 mM
2	phosphotyrosine peptide	natural abundance	0.8 mM
3	H2O	natural abundance	93 mM
4	D2O	natural abundance	7 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Release date

2011-02-08

Citation

1H, 13C and 15N backbone and side-chain chemical shift assignment of the Fyn SH2 domain and its complex with a phosphotyrosine peptide
Huculeci, R., Buts, L., Lenaerts, T., van Nuland, N.A.J.
Biomol. NMR Assign. (2011), 5, 181-184, PubMed 21298565 , DOI 10.1007/s12104-011-9295-4 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17368 released on 2011-02-08
Title Fyn SH2 free form

Related entities 1. FynSH2 correct, : 3 : 97 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCO