BMRBj - BMREntryMaster

Found 1 Document (1.5 seconds)

BMRB: 17373

8CN3

PDZ2 from human SAP97

Authors

Chi, C., Bach, A., Engstrom, A., Stromgaard, K., Lundstrom, P., Ferguson, N., Jemth, P.

Assembly

pdz domain

Entity

1. pdz domain (polymer, Thiol state: not present), 109 monomers, 11678.32 Da Detail

MHHHHHLVPR GSKPVSEKIM EIKLIKGPKG LGFSIAGGVG NQHIPGDNSI YVTKIIEGGA AHKDGKLQIG DKLLAVNNVA LEEVTHEEAV TALKNTSDFV YLKVAKPTS

Formula weight

11678.32 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.9 %, Completeness: 37.8 %, Completeness (bb): 72.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.8 % (470 of 1244)	15.1 % (97 of 644)	57.7 % (282 of 489)	82.0 % (91 of 111)
Backbone	72.0 % (464 of 644)	43.1 % (97 of 225)	87.9 % (277 of 315)	86.5 % (90 of 104)
Sidechain	12.5 % (87 of 697)	0.0 % (0 of 419)	32.1 % (87 of 271)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 34)	0.0 % (0 of 34)
Methyl	7.2 % (10 of 138)	0.0 % (0 of 69)	14.5 % (10 of 69)

1. SAP97 PDZ2 polypeptide

MHHHHHLVPR GSKPVSEKIM EIKLIKGPKG LGFSIAGGVG NQHIPGDNSI YVTKIIEGGA AHKDGKLQIG DKLLAVNNVA LEEVTHEEAV TALKNTSDFV YLKVAKPTS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	SAP97 PDZ2 polypeptide	[U-100% 15N]	0.7 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Release date

2011-01-13

Citation

Biophysical characterization of the complex between human papillomavirus E6 protein and synapse-associated protein 97
Chi, C.N., Bach, A., Engstrom, A., Strmgaard, K., Lundstrom, P., Ferguson, N., Jemth, P.
J. Biol. Chem. (2011), 286, 3597-3606, PubMed 21113079 , DOI 10.1074/jbc.M110.190264 , Abstract

Related entities 1. pdz domain, : 2 : 5 : 304 entities Detail

Interaction partners 1. pdz domain, : 100 interactors Detail

More details for 100 interactors identified by 31 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17373_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17373_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	457		88.1 (chain: 1, length: 109)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 109, Sequence coverage: 88.1%
- Total number of assignments
  - ¹H chemical shifts: 90
  - ¹³C chemical shifts: 277
  - ¹⁵N chemical shifts: 90
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	644	90	14.0
¹³C chemical shifts	489	277	56.6
¹⁵N chemical shifts	112	90	80.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	225	90	40.0
¹³C chemical shifts	218	192	88.1
¹⁵N chemical shifts	104	90	86.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	419	0	0.0
¹³C chemical shifts	271	85	31.4
¹⁵N chemical shifts	8	0	0.0

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Found 1 Document (1.5 seconds)

BMRB: 17373

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. pdz domain, : 2 : 5 : 304 entities Detail

Interaction partners 1. pdz domain, : 100 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample

Instrument

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Chemical shift validation 3 contents Detail