BMRBj - BMREntryMaster

Found 1 Document (0.34 seconds)

BMRB: 17413

2ZCZ

B. ste. A26I Holo TRAP Ile, Leu, and Val methyls

Authors

Kleckner, I.R.

Assembly

TRAP11

Entity

1. TRAP (polymer, Thiol state: not present), 74 monomers, 8284.332 × 11 Da Detail

MYTNSDFVVI KALEDGVNVI GLTRGIDTRF HHSEKLDKGE VLIAQFTEHT SAIKVRGKAY IQTRHGVIES EGKK

2. TRP (non-polymer), 1 monomers, 204.225 Da

Total weight

91331.88 Da

Max. entity weight

8284.332 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 28.4 %, Completeness: 14.0 %, Completeness (bb): 7.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	14.0 % (110 of 783)	12.6 % (56 of 445)	16.0 % (54 of 338)
Backbone	7.6 % (28 of 370)	6.5 % (10 of 155)	8.4 % (18 of 215)
Sidechain	18.3 % (88 of 480)	15.9 % (46 of 290)	22.1 % (42 of 190)
Aromatic	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)
Methyl	67.4 % (62 of 92)	67.4 % (31 of 46)	67.4 % (31 of 46)

1. TRAP

MYTNSDFVVI KALEDGVNVI GLTRGIDTRF HHSEKLDKGE VLIAQFTEHT SAIKVRGKAY IQTRHGVIES EGKK

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 8.0

#	Name	Isotope labeling	Concentration
1	TRAP	[U-2H; U-15N; ILV-(13CH3)]	1-1.5 mM
2	potassium chloride	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.02 %

Release date

2012-02-12

Citation

Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics
Kleckner, I.R., Gollnick, P., Foster, M.P.
J. Mol. Biol. (2012), 415, 372-381, PubMed 22115774 , DOI 10.1016/j.jmb.2011.11.019 , Abstract

Related entities 1. TRAP, : 1 : 28 : 11 entities Detail

Interaction partners 1. TRAP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17413_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17413_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	116		24.3 (chain: 1, length: 74)
1	Chemical shift references	chemical_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 74, Sequence coverage: 24.3%
- Total number of assignments
  - ¹H chemical shifts: 87
  - ¹³C chemical shifts: 29
- Completeness of assignments
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts

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Found 1 Document (0.34 seconds)

BMRB: 17413

Sample

Related entities 1. TRAP, : 1 : 28 : 11 entities Detail

Interaction partners 1. TRAP, : 1 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 4 contents Detail