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1H, 13C and 15N backbone chemical shift assignments of ubiquitin at pH 7.2
Authors
Lorenz, S.G., Wemmer, D.E., Kuriyan, J., Pelton, J.G.
Assembly
ubiquitin
Entity
1. ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG


Formula weight
8564.731 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.7 %, Completeness: 32.5 %, Completeness (bb): 61.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All32.5 % (297 of 913)18.0 % (86 of 478)40.0 % (140 of 350)83.5 % (71 of 85)
Backbone61.9 % (275 of 444)45.6 % (68 of 149)61.7 % (137 of 222)95.9 % (70 of 73)
Sidechain16.1 % (87 of 539) 5.5 % (18 of 329)34.3 % (68 of 198) 8.3 % (1 of 12)
Aromatic 0.0 % (0 of 32) 0.0 % (0 of 16) 0.0 % (0 of 16)
Methyl 4.1 % (4 of 98) 2.0 % (1 of 49) 6.1 % (3 of 49)

1. ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2


#NameIsotope labelingTypeConcentration
1ubiquitin[U-99% 13C; U-99% 15N]1640 uM
2sodium phosphatenatural abundance75mM mM
3D2Onatural abundance7.5 %
4DSSnatural abundance30 uM
5H2Onatural abundance92.5 %

LACS Plot; CA
Referencing offset: -0.07 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.07 ppm, Outliers: 4 Detail
Release date
2011-03-08
Citation
The mechanism of linkage-specific ubiquitin chain elongation by a single-subunit E2
Wickliffe, K.E., Lorenz, S., Wemmer, D.E., Kuriyan, J., Rape, M.
Cell (2011), 144, 769-781, PubMed 21376237 , DOI 10.1016/j.cell.2011.01.035 ,
Related entities 1. ubiquitin, : 1 : 200 : 5 : 25 : 140 entities Detail
Interaction partners 1. ubiquitin, : 27 interactors Detail
Experiments performed 3 experiments Detail
Keywords E2, K11-linked chain, substrate-assisted catalysis, ubiquitin, ubiquitination