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BMRB: 17471

4EH2

Backbone 1H, 13C, and 15N Chemical Shift Assignments for p38 alpha MAPK

Authors

Francis, D.M., Peti, W., Page, R.

Assembly

p38 alpha

Entity

1. p38 alpha (polymer, Thiol state: all free), 352 monomers, 40290.69 Da Detail

GHMGSQERPT FYRQELNKTI WEVPERYQNL SPVGSGAYGS VCAAFDTKTG LRVAVKKLSR PFQSIIHAKR TYRELRLLKH MKHENVIGLL DVFTPARSLE EFNDVYLVTH LMGADLNNIV KCQKLTDDHV QFLIYQILRG LKYIHSADII HRDLKPSNLA VNEDCELKIL DFGLARHTDD EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL TGRTLFPGTD HIDQLKLILR LVGTPGAELL KKISSESARN YIQSLTQMPK MNFANVFIGA NPLAVDLLEK MLVLDSDKRI TAAQALAHAY FAQYHDPDDE PVADPYDQSF ESRDLLIDEW KSLTYDEVIS FV

Formula weight

40290.69 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 34.5 %, Completeness (bb): 59.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	34.5 % (1445 of 4184)	21.9 % (474 of 2166)	41.8 % (689 of 1647)	76.0 % (282 of 371)
Backbone	59.5 % (1239 of 2084)	46.0 % (325 of 707)	61.0 % (634 of 1039)	82.8 % (280 of 338)
Sidechain	20.3 % (495 of 2435)	10.3 % (150 of 1459)	36.4 % (343 of 943)	6.1 % (2 of 33)
Aromatic	1.7 % (6 of 362)	1.7 % (3 of 181)	1.7 % (3 of 176)	0.0 % (0 of 5)
Methyl	16.9 % (73 of 432)	12.0 % (26 of 216)	21.8 % (47 of 216)

1. p38 alpha

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	p38_alpha	[U-13C; U-15N; U-2H]	0.58 mM
2	HEPES	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	p38 alpha	[U-2H;U-15N]	0.2 mM
8	HEPES	natural abundance	50 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.67 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.67 ppm, Outliers: 2 Detail

Release date

2011-12-11

Citation

Structural basis of p38α regulation by hematopoietic tyrosine phosphatase
Francis, D.M., Roycki, B., Koveal, D., Hummer, G., Page, R., Peti, W.
Nat. Chem. Biol. (2011), 7, 916-924, PubMed 22057126 , DOI 10.1038/nchembio.707 , Abstract

Related entities 1. p38 alpha, : 1 : 99 : 200 entities Detail

Interaction partners 1. p38 alpha, : 97 interactors Detail

More details for 97 interactors identified by 36 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC